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11 

 

OPE-1-BA-e-1610 

5.2  (Pre-) focussing 

 
When  you  are  observing  an  object,  you  must  have  the  correct  distance  to  the 
objective to achieve a sharp image.  
In  order  to  find  this  distance  at  the  beginning  (without  other  default  settings  of  the 
microscope) place the objective with the lowest magnification in the beam path, look 
through  the  right  eyepiece  with  the  right  eye  and  turn  it  slowly  using  the  coarse 
adjustment knob 

(see illustration)

 
 

 

 
 
The simplest way of doing this would be to first raise the specimen stage (using the 
coarse adjustment knob) until it is just under the objective and then lower it slowly. As 
soon as an image is recognisable (no matter how sharp), then you should only adjust 
the focus using the fine adjustment knob. 
 
 
Adjusting the torque of the coarse and fine adjustment knob 

 

Next to the left adjustment wheel for the coarse and fine adjustment knob there is a 
ring which you can use to alter the torque of these wheels. Turning it in a clockwise 
direction reduces the torque and turning it in an anti-clockwise direction increases it. 
On one hand, this function can help to make it easier to adjust the focus and on the 
other hand it can prevent the specimen stage from slipping down unintentionally. 
 
 
Important: 

 

In  order  to  avoid  damaging  to  the  focussing  system,  the  left  and  right  adjustment 
wheels for the coarse and fine adjustment  knob must  never be rotated at  the same 
time in opposite directions. 
 

 

Summary of Contents for OPE 118

Page 1: ...RN Sohn GmbH Ziegelei 1 D 72336 Balingen E Mail info kern sohn com Tel 49 0 7433 9933 0 Fax 49 0 7433 9933 149 Internet www kern sohn com User instructions Polarisation microscope KERN OPE 1 OPE 118 V...

Page 2: ......

Page 3: ...Features 7 4 Assembly 9 4 1 Polarisation unit Microscope head 9 4 2 Objectives 9 4 3 Eyepieces 9 4 4 Colour filter 10 4 5 Condenser Polariser 10 5 Operation 10 5 1 Getting started 10 5 2 Pre focussing...

Page 4: ...n additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The infor...

Page 5: ...microscope is not being used you should cover it with the enclosed dust protective cover When doing this the power supply is stopped by switching off at the main switch and unplugging the mains cable...

Page 6: ...these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However organi...

Page 7: ...OPE 1 BA e 1610 6 2 Nomenclature...

Page 8: ...e pieces Objectives Illumination OPE 118 Finite Monocular WF 10x 18 mm Achromatic 4x 10x 40x 6V 20W Halogen Transmitted Product dimensions 360x200x400 mm Packaging dimensions 440x340x240 mm Net weight...

Page 9: ...OPE 1 BA e 1610 8...

Page 10: ...should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures The Bertrand lens and the analyser are inherent parts of the polarization unit The...

Page 11: ...en you need to remove the condenser Then use the focus dial of the condenser to move the condenser holder to a low position In this way the condenser can be taken off after loosening the three screws...

Page 12: ...soon as an image is recognisable no matter how sharp then you should only adjust the focus using the fine adjustment knob Adjusting the torque of the coarse and fine adjustment knob Next to the left a...

Page 13: ...g on its angle position between polariser and analyser For getting ideal results the centre of the rotation axis of the table must be aligned to the centre of the optical beam path The microscopes of...

Page 14: ...If the table is not or heavy to revolve even though the fixing screw is loosened this serves as an evidence for a significant decentration of the table 6 If the table is perfectly centre adjusted you...

Page 15: ...objectives you should use the silver ring above the objectives see illustration When rotating the nosepiece you must always make sure that the objective which is about to be positioned in the beam pat...

Page 16: ...ure diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image For the objective with the lowest magnification the lever of the aperture diap...

Page 17: ...in the field of view The slide of the Bertrand lens needs to be in the pulled out position for standard polarisation processes It can be moved into the beam path in order to observe the interference...

Page 18: ...connection point of its cable Now the new module has to be mounted in the same why as the original one After the bulb holder has been replaced in the underside of the device and the screws replaced t...

Page 19: ...y centred The condenser is too low The field of view is dark or is not correctly illuminated The objective is not positioned correctly on the beam path The selector switch for the beam path is between...

Page 20: ...Dirt dust on the front lens of the condenser Dirt dust on the object One side of the image is blurred The stage was not correctly fitted The objective is not positioned correctly on the beam path The...

Page 21: ...materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in force...

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