KERN OBN 141, User Instructions

Page 1: ...hn com Tel 49 0 7433 9933 0 Fax 49 0 7433 9933 149 Internet www kern sohn com User instructions Fluorescence microscope KERN OBN 14 OBN 141 OBN 147 OBN 148 Version 1 0 01 2015 Import r Impex Produkter...

Page 2: ......

Page 3: ...ndenser 13 5 Operation 13 5 1 Getting started 13 5 2 Pre focussing 14 5 3 Adjusting the interpupillary distance 15 5 4 Dioptre adjustment 15 5 5 Adjusting the magnification 16 5 6 Adjusting the Koehle...

Page 4: ...n additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The infor...

Page 5: ...ich are too high or too low vibrations dust or a high level of humidity The ideal temperature range is between 0 and 40 C and a relative humidity of 85 should not be exceeded The device should always...

Page 6: ...s these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However organ...

Page 7: ...d into two main components Compound microscope KERN Professional Line Fluorescence reflected light unit The following user manual refers at first merely to the compound microscope The disquisition on...

Page 8: ...erture adjustment Condenser Condenser Field lens with Field diaphragm Control dial X Y axis Specimen stage Fine adjustment knob Coarse adjustment knob Dimmer Tube connectors with dioptre adjustment ri...

Page 9: ...OBN 14 BA e 1510 8 Rear view Adjusting ring torque Carrying handle Main switch Power connection Coarse adjustme nt knob Fine adjustment knob Fuse Condenser Focus dial...

Page 10: ...B G OBN 147 Infinity Trinocular Halogen 100W Epi Fluorescence B G OBN 148 Infinity Trinocular Halogen 100W Epi Fluorescence B G UV V Eyepieces WF 10x 20 mm Objectives 4x 10x 20x 40x 100x Product dime...

Page 11: ...OBN 14 BA e 1510 10...

Page 12: ...11 OBN 14 BA e 1510 4 Assembly...

Page 13: ...ongest magnification appears You must make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures For objectives which are marked OIL you must use an immers...

Page 14: ...ccessories 5 Operation 5 1 Getting started The very first step is to establish a power connection using the mains plug You should first adjust the dimmer to a low level so that when you look through t...

Page 15: ...soon as an image is recognisable no matter how sharp then you should only adjust the focus using the fine adjustment knob Adjusting the torque of the coarse and fine adjustment knob Next to the left a...

Page 16: ...llary distance is set correctly 5 4 Dioptre adjustment The eye strengths of each eye of the microscope user can often be slightly different which in daily life has no consequences But when using a mic...

Page 17: ...objectives you should use the silver ring above the objectives see illustration When rotating the nosepiece you must always make sure that the objective which is about to be positioned in the beam pat...

Page 18: ...hragm When adjusting the Koehler lighting for the first time you must first select the lowest possible objective magnification so that you can carry out the following steps 1 Use the condenser focus w...

Page 19: ...he condenser up so high that it collides with the object holder Therefore care is needed when doing this 4 Use the centring screws of the condenser holder to bring the image from the field diaphragm i...

Page 20: ...3 of the pupil diameter If the eyepiece should be removed for checking then please make sure that no dirt or dust falls into the tube 7 It is possible to alter the brightness of the bulb using the di...

Page 21: ...sses symbol on the side They are also marked in the item description by an additional H example HSWF 10x 23 mm When fitting the eye cups make sure that the dioptre setting is not moved We would theref...

Page 22: ...Lower the specimen stage and position the 100x objective in the beam path 3 Bring the specimen stage or object slide to the objective very slowly until there is slight contact 4 Observe the object Th...

Page 23: ...tained in the spectrum of the light source HBO or LED The excitation filter is only permeable for the according wave length After that the excitation light hits a dichroic mirror which reflects it tow...

Page 24: ...23 OBN 14 BA e 1510 Overview of wave lengths for excitation and emission per excitation filter Blue line Wave length excitation Green line Wave length emission...

Page 25: ...OBN 14 BA e 1510 24...

Page 26: ...25 OBN 14 BA e 1510...

Page 27: ...er for FL module Fixing Allen screw for microscope head Main body Connection point for microscope head Fixing screw for lamp housing 7 8 9 10 11 HBO LED lamp housing Power connection Screw for attachi...

Page 28: ...device for field diaphragm Control lever for field diaphragm Centring device for aperture diaphragm Control lever for aperture diaphragm 6 7 8 9 10 Condenser control Screw for cover of lamp housing Ce...

Page 29: ...fluorescence reflected light units available for the microscopes of the series OBN 14 They have the following specifications Article number Illumination Excitation filter Centring objective Input volt...

Page 30: ...he reflected light unit for special fluorescence applications it will be favourable to adjust the microscope in the bright field mode This includes the placing of the sample adjusting the interpupilla...

Page 31: ...reduction of the lamps life time Equally the lamp must not be switched on directly after a switch off process When pausing the observation you should always slide in the control lever for the illumin...

Page 32: ...Use the two centring screws on the lamp housing for moving the light spot into the centre of the hair cross Warning Heat development on the lamp housing Changing the lamp HBO 1 Disconnect the FL refl...

Page 33: ...he mains power supply socket With the device switched off and unplugged you can screw out the housing The defective fuse can be removed from its housing and be replaced with a new one After that you j...

Page 34: ...lb can be pulled out of the socket and be replaced with a new one After the bulb holder has been replaced in the underside of the device and the screws replaced the bulb replacement procedure is compl...

Page 35: ...ece see illustration on page 10 where you can simply insert the analyser see left illustration The polariser on the other hand is only placed on the ring holder of the field lens see right illustratio...

Page 36: ...ng the microscope camera i e using the PC screen which is connected To do this the trinocular toggle rod on the righthand side of the microscope head must be pulled out The light from the microscope l...

Page 37: ...This consists of a simple PH condenser a PH objective with a specific magnification 10x 20x 40x or 100x a PH slider which is adapted to the lens being used a centring telescope and two green filters...

Page 38: ...es of the user using a green filter can produce a more effective and pleasant image To achieve this the green filter must be screwed onto the underside of the PH condenser 2 Comprehensive phase contra...

Page 39: ...a black objective ring The black one is central and the white one possible is pushed to one side see left illustration g Now press the turnscrews fitted on the side of the condenser towards the centr...

Page 40: ...ly centred The condenser is too low The field of view is dark or is not correctly illuminated The objective is not positioned correctly on the beam path The selector switch for the beam path is betwee...

Page 41: ...Dirt dust on the front lens of the condenser Dirt dust on the object One side of the image is blurred The stage was not correctly fitted The objective is not positioned correctly on the beam path The...

Page 42: ...y materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in force...

Page 43: ...______________________________________________________ __________________________________________________________ __________________________________________________________ ___________________________...