OBN-14-BA-e-1510
30
Control elements for illumination
The following control elements for the illumination play a role within the fluorescence
microscopy:
Field diaphragm, aperture diaphragm, condenser:
Optimisation of the contrast and the light yield.
Control lever for illumination:
When located at the medium position there is a filter, which softens the
lighting, so that samples, which are not stable enough for the full illuminating
power, can be observed as well.
Important warnings for the usage of a HBO lamp
During operation the lamp is showing a strong heat development. It should
be avoided to come into contact with the lamp housing while and after
some time of running the reflected light unit.
The lamp must not in any circumstances be switched off during the pre-
heating. It could cause a significant reduction of the lamps life time.
Equally the lamp must not be switched on directly after a switch-off
process.
When pausing the observation you should always slide in the control lever
for the illumination in order to interrupt the light beam. The light spectrum of
the HBO lamp often can be harmful for microbes.
Never look inside the eyepieces when the beam path is opened (by the
control lever for illumination) and there is set an empty position of the FL
module at the same time. There is the risk of blinding.
A HBO lamp has a certain life time. The closer you push it to its limit, the
bigger the risk of an explosion of the lamp and setting free of toxic mercury
vapour. This must be avoided at all costs.
The following devices can provide information about the necessity of a
lamp replacement (This applies for 100W HBO lamps):
o
Ampere meter on the power supply unit
As soon as 4.8 A are reached
lamp replacement
o
Life time display on the power supply unit
As soon as 100 h are reached
lamp replacement
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