Knauer ADA01XA, Instructions Manual

Page 1: ...HPLC Detector UVD 2 1L Instructions Document No V6831 ...

Page 2: ...x 49 30 8015010 Internet www knauer net E Mail info knauer net Document number V6831 Version number 4 0 Release date 2019 12 05 Original edition The information in this document is subject to change without prior notice For the latest version of the instructions visit our website www knauer net library For the printed versions of our instructions we use environmentally friendly paper from sustaina...

Page 3: ... safety equipment 4 2 5 Safety features on the device 4 2 6 Working with solvents 5 2 6 1 General requirements 5 2 6 2 Contamination by health threatening solvents 5 2 6 3 Avoiding leakage 5 2 7 Specific environments 6 2 7 1 Earthqake endangered areas 6 2 7 2 Explosive environment 6 2 7 3 Cooling room 6 2 8 Maintenance care and repair 6 2 9 Service request form and decontamination report 6 3 Produ...

Page 4: ...ontrol Setting a dynamic IP address via device serial number 21 4 9 4 Firmware Wizard Setting a static IP address 22 4 9 5 Firmware Wizard Setting a dynamic IP address 23 4 10 Remote control 24 4 10 1 Connector assignment 24 4 10 2 Analog control 26 4 10 3 Integrator connector 26 4 10 4 Connecting the pin header 26 4 11 Switching on 27 5 Operation 28 5 1 Checklist before operation 28 5 2 Software ...

Page 5: ...g the device 42 10 Disposal 43 10 1 AVV Marking Germany 43 10 2 WEEE registration number 43 10 3 Eluents and other operating materials 43 11 Technical data 44 11 1 Detection 44 11 2 Communication 44 11 3 General 45 12 Reorders 45 12 1 Devices 45 12 2 Software 46 12 3 Accessories and spare parts 46 12 4 Fiber optic connectors 46 12 5 Flow cells 46 12 5 1 Analytical flow cells 46 12 5 2 Preparative ...

Page 6: ...se and other instructions from the KNAUER web site www knauer net library 1 2 Signal words Possible dangers related to the device are distinguished in personal and material damages Symbol Meaning DANGER red indicates a highly hazardous situa tion If not avoided it will result in death or serious injury WARNING orange indicates a hazardous situa tion If not avoided it could result in death or serio...

Page 7: ...e In case you notice any damage inform the Technical Support and the shipping company within three workdays 1 4 3 Warranty conditions For information on warranty please refer to our general terms and condi tions on the website www knauer net terms 1 4 4 Warranty seal A blue or orange warranty seal is affixed to some devices A blue seal is used by KNAUER s Manufacturing or Customer Support for devi...

Page 8: ...guarantee the functionality and safety of the device Operation in potentially explosive areas without special and additio nal explosion protection Contact the KNAUER Customer Support for more information 2 2 User qualification The user is qualified to handle the device if all of the following points apply He has at least a basic knowledge of liquid chromatography He has knowledge about the propert...

Page 9: ...bout the dangers Provide the necessary safety equipment to the employees working with the unit see section below 2 4 Personal safety equipment The protective measures required in the laboratory must be observed and the following protective clothing worn during all work on the device Safety glasses with side protection Protective gloves in accordance with the prevailing ambient conditions and used ...

Page 10: ...h threatening solvents Contamination with toxic infectious or radioactive substances poses a hazard for all persons involved during operation repair sale and dis posal of a device All contaminated devices must be properly decontaminated by a spe cialist company or the operating company before they can be recom missioned repaired sold or disposed see 10 Disposal p 43 2 6 3 Avoiding leakage Risk of ...

Page 11: ...ls recommended or prescribed by the manufacturer Spare parts and accessories Only use original parts and accessories made by KNAUER or a company authorized by KNAUER PEEK fittings Use PEEK fittings only for a single port or brand new PEEK fittings in order to avoid dead volume or not exactly fitting connections Column care Follow KNAUER or other manufacturer s instructions on caring for the column...

Page 12: ... 3 1 2 Flow cells Various types of flow cells are available to the user Pay attention to the compatibility of the flow cells Note A test cell is preassembled to the detector Before the detector can be used for measurements the test cell has to be replaced by a flow cell The fiber optics version of the detector is delivered without a test cell 3 2 Scope of delivery The following items should be inc...

Page 13: ...ws Legend 1Leak tray 2Capillary guides 3Standby switch 4Status LEDs 5Flow cell 5 4 2 2 2 1 3 Fig 1 Front view Legend 1Serial number label 2Integrator output 3LAN port 4RS 232 port service only 5Pin header 6Fan 7Power connection 8Power switch 2 3 4 6 5 Fig 2 Rear view 1 8 7 ...

Page 14: ... message Green Device is measuring center LED Off Device is not calibrated or lamp is off Start calibration via soft ware or via rebooting the device Switch on the lamp via the software Flashes green Device is calibrating Wait until the device is ready Green Device is ready for operation right LED Green Device is active Blue Standby Exit standby by pressing the standby switch To start the standby ...

Page 15: ...r Obey maximum load for leak tray during transpor tation installation and operation A device marked with CE fulfills the product spe cific requirements of European directives This is confirmed in a Declaration of Conformity Testing seals in Canada and the USA at nationally recognized testing centers NRTL The certified device or system has successfully passed the qual ity and security tests A warra...

Page 16: ...parts are loosely attached to the device Keep the included packing list for repeat orders Keep the original packaging for safe storage or transportation 4 2 Ambient conditions 4 2 1 Operation site Observe the following requirements for the operation site so that the measurement results are not influenced Place on a firm level and straight surface Protect against direct sunlight Do not expose to ai...

Page 17: ...ower of the connected devices must not exceed 50 of the highest connected power capacity since higher currents can flow briefly when the device is switched on Main connection The electrical power supply at the operation site must be connected directly to the nearest main power connection Grounding The connectors for the voltage must be grounded accordingly Power supply cables and plugs Original pa...

Page 18: ...d Procedure Process Figure 1 Carefully push the funnel 1 into the center opening of the capillary guide 2 1 2 Fig 3 Funnel and capillary guide 2 Push the long ending of the first nozzle 4 into the hose 3 3 4 Fig 4 Hose and nozzle 3 Afterwards push the nozzle onto the funnel 4 Push the other end of the hose onto the nozzle 5 of the leak tray 5 Fig 5 Hose connected to device 5 Attach the waste nozzl...

Page 19: ...r plug is disconnected from the device Steps Figure 1 Unscrew the 2 knur led head screws 1 Fig 7 Removing the slide 2 Pull out the slide 2 3 Remove the test cell 4 Insert the flow cell 3 into the slide Fig 8 Assembling the flow cell 5 Push the slide into the detector 2 6 Insert the knurled he ad screws and screw tight The flow cell is assembled Connect the capillaries Prerequisite Procedure 1 3 2 ...

Page 20: ...cell Flow cell is removed from the detector Allen screwdriver size 3 Process 1 Using the allen screwdriver loosen the threaded ring 1 2 Remove the cover 2 3 To shorten the optical path length remove the spacer 3 To enlarge the optical path length put in one or both spacers 4 Insert the cover 2 5 Using the allen screwdriver tighten the threaded ring 1 The optical path length is set Mount the flow c...

Page 21: ...ing 1 Fig 10 Capillary fitting 2 Push the clamping ring 3 onto capillary 3 Manually screw toge ther the capillary 5 and the flow cell 4 Tighten the fitting with the wrench Fig 11 Capillary and flow cell The capillary is connected Component defect Damage to components due to excessive tightening possible Observe the torque of the screw connection Use 5 Nm torque for stainless steel fittings Use 1 N...

Page 22: ...et the LAN properties see section 4 8 2 4 8 2 Configuring the LAN settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address In Windows power saving hibernation standby and screen saver must be deactivated In case you use an USB to COM box the option Allow the computer to turn off this device to save power in the device manager m...

Page 23: ...r 1 2 3 4 5 Abb 12 Connect devices with LAN The computer is switched off There is a patch cable for each device and the computer Process 1 Use the patch cable to connect the router and the computer Re peat this step to connect all devices 2 Use the power supply to connect the router to the mains power system Set the router properties see section 4 8 4 4 8 4 Configuring the router The router is pre...

Page 24: ...nto the company network 4 8 6 Controlling several systems separately in LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one chromatography systems are connec ted to the same LAN and you plan on controlling them separately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same n...

Page 25: ...lications More information about LAN settings can be found in the Mobile Control Software Instructions in the chapter Device Settings document no V6851 The device is switched on Mobile Control is installed and running The connection between Mobile Control and the device has been established Process 1 In Mobile Control click Settings 2 On the General tab choose the device name 3 In Network Settings...

Page 26: ... 14 Network settings for dynamic IP address The device is now accessible via a dynamic IP address 4 9 3 Mobile Control Setting a dynamic IP address via device serial number The device is switched on Mobile Control is installed and running The connection between Mobile Control and the device has been established Process 1 In Mobile Control click Settings 2 In Network Settings click Reset The window...

Page 27: ... 2 Setting IP address manually 3 Text box for IP address 4 Text box for subnet mask gateway 5 Confirm changes Abb 16 Network settings in Firmware Wizard The device is switched on Firmware Wizard is installed and running The connection between Firmware Wizard and the device has been established Process 1 In Firmware Wizard click Reset LAN Settings 2 The window Device connection settings opens Enter...

Page 28: ...c IP address The device is switched on Firmware Wizard is installed and running The connection between Firmware Wizard and the device has been established Process 1 In Firmware Wizard cleck Reset LAN Settings 2 The window Device connection settings opens Enter serial num ber of the device into the text field Target device serial number 1 3 Select option Obtain an IP address automatically 2 4 Click...

Page 29: ...r assignment Plug Function EV 1 Event 1 Relay contact The contact is on a floating basis Its setting de pends on the settings in the software Steady rate signal passive open relay contact active closed relay contact Pulse closed relay contact for at least 1000 ms permissible load of the relay contact 1 A 24 V DC EV 2 Event 2 TTL compatible output Levels passive 5 V active 0 V Pulse 0 V for at leas...

Page 30: ...sent via LAN Autozero Low active Secure switching threshold at least 10 mA A signal short circuit to ground sets the measuring signal to zero 5V Provides a voltage of 5 V with respect to GND This makes it possible to supply an appliance that is switched by an EVENT Max current 50 mA GND Reference point of the voltage at the signal inputs 24V Valve Event controlled switching of 24 V against GND Max...

Page 31: ...g signals from the detector non bipolar 1 channel 0 to 5 V DAC 20 bit scalable adjustable to offset 4 10 4 Connecting the pin header To control one device through another the pin header is used To use remote control you have to connect cables to the pin header The single ports are used to exchange control signals The device is switched off The power plug is disconnected from the device Operating t...

Page 32: ...ng the device into operation 4 11 Switching on The device is switched on by the power key on the back of the device Installation has been completed Flow cell is mounted on the detector Steps Figure 1 Insert the plug into the female connector 2 at the rear panel Fig 19 Power switch with female connector 2 Plug the other end into an electric socket 3 Switch on the device at the power switch 1 The de...

Page 33: ...trol A device can be operated in several ways with chromatography software with Mobile Control Note It is not possible to use two operating methods simultaneously If the device is connected to the software it cannot be controlled via Mobi le Control The device status can however be monitored 5 2 1 Chromatography software To control the device with chromatography software it must be connec ted to t...

Page 34: ...evice Using the Mobile Control you can re set the detector to its default settings Parameter Settings Network LAN DHCP port 10001 Lamps D2 ON Time constant 2 s Channels Channel 1 WL 254 nm Analog out Offset 1 10 mV Scale 1 1 AU V Event check All events deactivated o Date Time Current date time Fraction collector OFF Leak sensor ON Sensitivity low ...

Page 35: ...alification OQ The Operation Qualification includes an extensive functionality test accor ding to KNAUER standard OQ documents The Operation Qualification is a standardized document and free of charge It is not part of the delivery Please contact the Technical Support in case of request The Operation Qualification includes the following definition of customer requirements and acceptance terms docu...

Page 36: ...uter 2 Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router correct Are the cables plugged in tightly 4 If the router is integrated into a company network pull out the patch cable from...

Page 37: ...incursion from interfe ring light or an electronics error 2 Insert the test cell 3 Inspect the calibration with a weak ab sorbing eluent Baseline noise 1 Inspect the flow cell assembly 2 Fasten the knurled head screws on the slide to prevent incursion from interfe ring light or an electronics error 3 Exchange the defective flow cell 4 Inspect the service life of the lamp on the display 5 Reduce th...

Page 38: ... have enough light for the calibrat ion due to for instance a defective lamp Restart the device Calibration failed Signal path open Restart the device Calibration failed Too much light Restart the device Cannot delete active program link 1 Pause link 2 Delete program Cannot edit program from the running link 1 Pause link 2 Edit data using chromatography software Cannot initialize LAN Check cables ...

Page 39: ...r Support Data acquisition active No entries are possible Stop acquiring measurement data then you can make a new entry Filter move error Restart the device GUI communication failed Restart the device Instrument remote controlled This entry is not executable Close and exit the software Invalid command 1 Check the cable connections 2 Change the entry Invalid line number Change the entry in the prog...

Page 40: ...f 50 program lines are possible Check the number of program lines Operation time out Restart the device Program does not exist Create a program Program is running Quit program or wait until program has been completed This link is used in WAKEUP 1 Quit or delete wakeup program wu Wake Up 2 Edit or delete link This program is used in a link 1 Pause or delete the link 2 Edit or delete data by means o...

Page 41: ...the flow cell or fiber optic cables Switch off the detector and disconnect it from the power supply Electronic defect Performing maintenance tasks on a switched on device can cause da mage to the device Switch off the device Pull the power plug Users may perform the following maintenance tasks themselves Regularly check the light intensity of the D2 lamp best before 2000 operating hours Inspect the ...

Page 42: ...er plug is disconnected from the device Capillaries are disconnected Steps Figure 1 Unscrew the knur led headscrews 1 Fig 20 Removing the slide 2 Pull out the slide 2 3 Remove the flow cell 4 Insert the flow cell 3 into the slide Fig 21 Assembling the flow cell 5 Push the slide into the detector 2 6 Insert the knurled head screws and screw tight The flow cell is assembled Connect the capillaries D...

Page 43: ...ow cell The following solvents are recommended for rinsing Diluted HCl 1 mol L 1 ml L NaOH aq Ethanol Acetone Syringe Procedure Process 1 Fill the syringe with eluent 2 Inject it into the inlet of the flow cell and allow it to act for 5 minutes 3 Fill the syringe with water and inject again 4 Use a nitrogen stream to dry the flow cell The flow cell is rinsed Check if the baseline noise has improve...

Page 44: ...pping on a clean surface remove the compression part 2 3 The lens 3 is protected by a seal ring 4 This must be renewed every time the lens is disassembled 4 Remove the lens and clean with a clean soft cloth or with water in an ultrasonic bath 5 Assemble the flow cell Make sure that the new seal ring does not interrupt the light path 6 Using the allen screwdriver tighten the threaded ring 1 The flo...

Page 45: ...cer 3 not part of all flow cells 3 Using tweezers pull out the compression bushing 4 and the light guide 5 4 Carefully push the light guide out of the holder and strip off the seal The seal ring needs to be renewed during every cleaning of the fiber optics 5 Clean the light guide with a clean soft cloth or with an appropriate solvent in an ultrasonic bath Make sure that the clean light guide is no...

Page 46: ... fiber optics with care and avoid impacts Move the fiber optics carefully without using pressure or bending it Detector is switched off Cap fittings for fiber optics Steps Figure 1 To detach from the flow cell unscrew the fittings of the fiber optics manu ally 1 Fig 24 Fiber optics fittings on flow cell 2 Seal the fiber optic con nectors with caps 2 Fig 25 Cap for optic connector 3 Manually unscre...

Page 47: ...the packaging Do not hold onto front cover or leak tray as these parts are loosely attached to the device 9 3 Transporting the device Documents If you want to return your device to KNAUER for repairs enclose the Service Request Form and Decontamination Report which can be downloaded from our website www knauer net servicerequest Device data For a secure transport note the weight and dimensions of ...

Page 48: ...er classifies to category 8 and 9 which among others comprises laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End users can send their old devices manufactured by KNAUER back to the distributor the importer or the company free of charge but would be charged for the disposal 10 3 Eluents and other operating materi...

Page 49: ...7 98 Fiber optics version 2 0 x 10 5 AU at 254 nm ASTM E1657 98 Drift 3 0 10 4 AU h at 254 nm ASTM E1657 98 Fiber optics version 4 0 x 10 4 AU h at 254 nm ASTM E1657 98 Linearity 2 0 AU at 270 nm ASTM E1657 98 Time constraints 0 0 0 1 0 2 0 5 1 0 2 0 5 0 10 0 s Integration time Automatic 11 2 Communication Max data rate 50 Hz LAN 20 Hz analog Interfaces LAN RJ 45 RS 232 SUB D 9 service only Pin he...

Page 50: ... C 39 2 104 F Air humidity below 90 non condensing 12 Reorders The list of repeat orders is current at the time of publication Deviations at a later time are possible Use the enclosed packing list for reordering spa re parts If you have any questions regarding spare parts or accessories please contact our Customer Support Current information on spare parts and accessories can be found on the Inter...

Page 51: ...RA accessories kit FZA02 Deuterium lamp A5193 12 4 Fiber optic connectors Name Order no 2 fiber optic connector 750 mm A0740 2 fiber optic connector 750 mm high temperature up to 85 C A0740HT 2 fiber optic connector custom made size A0743 12 5 Flow cells Most flow cells are also available equipped with fiber optic connectors for the use with the fiber optics version of the detector 12 5 1 Analytic...

Page 52: ... Material Max flow rate Max pressure 0 5 1 25 2 mm 1 8 2 3 mm 1 7 4 3 6 8 μl Stainless steel 1000 ml min 200 bar A4066 Fiber optics version A4078 Path length Connection Inner diameter Volume Material Max flow rate Max pressure 0 5 1 25 2 mm 1 8 2 3 mm 1 7 4 3 6 8 μl PEEK 1000 ml min 100 bar A4067 Fiber optics version A4079 Path length Connection Inner diameter Volume Material Max flow rate Max pre...

Page 53: ...bar A4152 1 Path length Capillary connection Material Max pressure 0 5 1 25 2 mm 1 2 with Tri Clamp Stainless steel 80 bar A4154 Path length Capillary connection Material Max pressure 0 5 1 25 2 mm 3 4 with Tri Clamp Stainless steel 80 bar A4155 Path length Capillary connection Material Max pressure 0 5 1 25 2 mm 1 4 with Tri Clamp Stainless steel 80 bar A4152 Path length Capillary connection Mate...

Page 54: ...therwise Please note that higher temperatures can signifi cantly affect the stability of different materials 13 2 Plastics Polyetheretherketone PEEK PEEK is a durable and resistant plastic and next to stainless steel the standard material in HPLC It can be used at temperatures up to 100 C and is highly chemical resistant against almost all commonly used sol vents in a pH range of 1 12 5 PEEK is po...

Page 55: ...tands out due to its high stiffness low friction value and thermic stability It can even substitute metal in many cases POM H TF is a combination of PTFE fibres and acetal resin and is softer and has better slip properties as POM The material is resistant against diluted acids pH 4 as well as diluted lyes aliphatic aromatic and halogenated hydrocarbons oils and alcohols It is not compatible with c...

Page 56: ...eramic is resistant against corrosion and wear and is fully biocompati ble An incompatibility against acids alkalis and solvents commonly used in HPLC is not known Alumina Al2O3 Due to their high resistance to wear and corrosion alumina ceramic is used as a coating for mechanically stressed surfaces It is a biocompatible material with low thermal conductivity and low thermal expansion Zirconium ox...

Page 57: ...iological appli cations which are metal ion sensible and applications with extreme cor rosive conditions These steels in comparison to commonly used steels are increasingly resistant against hydrochloric acid cyanides and other halogen acids chlorides and chlorinated solvents The use in ion cromatography is not recommended In case of electro chemical applications a passivation must be executed fir...

Page 58: ...14 replacing 37 Flushing solution 42 Foreseeable misuse 3 G GLP 45 I Initial startup 11 Inputs 44 Installation 11 Installation Qualification IQ 30 Integration time 44 Integrator connector 26 Intended use 3 Interfaces 44 IP address setting 20 23 L Lamp 4 36 LAN connecting 17 19 Troubleshooting 31 Leakage avoiding 5 management 13 Leak sensor 45 LED panel 9 Legal information 2 Liability limitation 2 ...

Page 59: ...lity 49 disposal 43 health threatening 5 Space requirement 12 Specific environments 6 Spectral bandwidth 44 Standby 9 Storage 42 Switching on 27 Symbols and signs 10 T Taking out of operation 42 Technical data 44 Temperature 45 ambient 11 Time constraints 44 Transport 42 damage 2 Troubleshooting 31 U Unpacking 11 User qualification 3 V Views 8 W Warranty conditions 2 Warranty seal 2 Wavelength set...

Page 60: ...AUER instructions online www knauer net library KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www knauer net KNAUER 2019 ...