1.
Loosen the thumb screw (1) to remove the condenser. Replace the
condenser with the new Abbe condenser provided in the phase kit.
2.
Keep printed face of slider towards you and two index slots an rear side
such that printing is straight readable. Remove left hand thumbscrews of
of phase slider and insert gently into slider slot provided in Abbe condenser
right end. Such that when 10x phase is legible outside phase ring is in the
field of view
3.
To ready phase slider for the use, gently push it into slider aperture provided
in the Abbe condenser as shown in Fig.1 with all legible text and part
number facing you. If inserted upside down or the wrong side facing you,
phase rings may be out of line causing distortion in image. Once in place,
re-tighten both thumbscrews to secure slider.
4.
Remove standard objectives from objective turret and fix 10X and 40X
phase objectives in place. Objectives other than phase contrast objectives
do not contain phase rings, and therefore will not produce a phase object.
5.
Remove blue filter from its seat on the collector lens and replace it with the
green filter provided with the phase kit.
1.
Turn microscope On and close the diaphragm of the Abbe condenser to
minimum position by shifting the condenser lever to control the amount
of collected light hitting condenser lens.
2.
Next engage the bright field ring by pushing the slider all the way in
and open the condenser diaphragm to a comfortable level of light by
adjusting condenser level. Place any specimen slide on stage and focus
the specimen with the 10X phase objective. Remove one eyepiece and
insert the centering telescopic eyepiece in its place as shown in Fig.2.
3.
Loosen the thumbscrew of the telescopic eyepiece. While looking into
the telescopic eyepiece, extend the front lens until the dark ring of 10X
objective in the field of view becomes as sharp as possible (more detail
is given on next page). Tighten the telescopic eyepiece’s thumbscrew
and image will remain in focus (Fig 3).
4.
Pull the phase slider out one click stop to bring the 10X ring into position
(you should be able to read 10X on the front of the slider).
5.
Two holes are provided on the front side of Abbe condenser for centering
the phase annulus by use of the two allen keys provided as shown in Fig
4.
INSTALLATION
SET UP FOR PHASE CONTRAST MICROSCOPY
Fig. 1
Fig. 2
Fig. 3
Fig. 4
