Focusing Procedure (Figure 30)
1.
Rotate the coarse adjustment knob (1) clockwise so that the
objective (3) is as close as possible to the specimen (We
recommend starting with 10X).
2.
While observing the specimen through the eyepieces, slowly rotate
the coarse adjustment knob (1) counterclockwise to lower the
stage.
3.
When coarse focusing of the specimen is obtained (an image is
observed), rotate the fine adjustment knob (2) for fine detail
focusing.
Working Distance (WD)
The WD refers to the distance between each objective and the specimen,
when acute focus of the specimen is obtained.
2
Adjusting the Focus
Fig. 30
1
2
3
WD
The inter-pupillary distance adjustment consists of regulating the two
eyepieces to align with both eyes’ pupils so that you can observe a single
microscopic image through two eyepieces in stereo vision. This greatly
helps to reduce fatigue and discomfort during observation.
While looking through the eyepieces, move both eyepieces laterally until
the left and right fields of view coincide completely. The position of index
dot (•) indicates the inter-pupiliary distance value.
Note your interpupillary distance so that it can be quickly referred to in the
future. This happens when multiple users work with the microscope.
3
Adjusting the Interpupillary Distance (IPD)
Fig. 31
Objectives
Eye Piece
Flip Top Abbe
10x/20 W.F. (4140010) Condenser
Objective
N.A. W.D. Cover Resolution Total Field of Depth of Obj.
Flip Position
Magnification
(mm) Glass ( m) mag. view/mm focus( m) N.A. (In / Out)
2.5x (9124002) 0.08 20.0 0.17 5.0 25x 8.0 400 0.08 Out
4x (9124005) 0.10 30.0 0.17 3.36 40x 5.0 200 0.10 Out
10x (9124010) 0.25 4.04 0.17 1.34 100x 2.0 30 0.25 Out
20x (9124010) 0.45 1.10 0.17 0.75 200x 1.0 6 0.45 In
40x (9124010) 0.65 0.45 0.17 0.52 400x 0.50 3 0.65 In
100x (oil) (9124010)
1.25 0.14 0.17 0.27 1000x 0.20 0.70 1.25 In
22
