Labomed TCM 400, User Manual

Page 1: ...ted on April 2022 TCM 400 Inverted Tissue Culture Microscopy User Manual To ensure proper use of this instrument as well as to avoid injury while operating Instrument understanding this manual completely before use is highly recommended ...

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Page 3: ...INOCULAR 6 5 TCM 400 ERGONOMIC 7 6 UNPACKING YOUR MICROSCOPE 8 7 STANDARD COMPONENTS 9 8 OPTIONAL ACCESSORIES 10 11 9 INITIAL SETUP ASSEMBLY 12 16 10 SUMMARY OF BRIGHT FIELD OBSERVATION PROCEDURE 17 11 DETAILED OBSERVATION PROCEDURE 18 19 12 TROUBLESHOOTING GUIDE 20 13 SPECIFICATIONS 21 TCM 400 ...

Page 4: ...of the design an aesthetically configured TCM400 is the instant choice for Culture studies in the era of bio sciences Offered with Infinity Corrected True color optical system choices of observation tubes soft feel turret movement stage extenders micro manipulators giving a paramount performance The user should be aware that the protection provided by the equipment may be impaired if used with acc...

Page 5: ...ential electrical hazards when replacing either halo gen bulb turn the microscope main switch to the OFF posi tion and disconnect power cord from wall outlet in advance Whenever you replace your microscope bulb allow lamp socket and bulb to cool before touching Fig 1 Applicable bulb replacement 6V30W Halogen bulb P N EL 455 3 Install microscope on a sturdy level table or bench and avoid any restri...

Page 6: ...o keep these chemicals away from open flames or potential Sources of electrical sparks For Example electrical equipment that is being switched ON or OFF Also remember to always use these chemicals only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass compo nents To clean non glass components use a lint free soft cloth slight...

Page 7: ...le dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab 5 To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfac tory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol 6 Grease must always be re...

Page 8: ...ation intensity control knob Main Switch Revolving Nose piece Binocular head Stage plate Illumination housing Lamp Housing Specimen holder Phase contrast slider Eyepieces TCM 400 BINOCULAR 5 TCM 400 3 7125000 795 TCM 400 Issue 1 3 Printed on April 2022 ...

Page 9: ... Main Switch Coarse and fine focus knob Revolving Nose piece Trinocular Port Stage plate Specimen holder Illumination housing Lamp Housing Phase contrast slider Eyepieces 6 TCM 400 TCM 400 TRINOCULAR 4 7125000 795 TCM 400 Issue 1 3 Printed on April 2022 ...

Page 10: ...gonomic head Stage plate Specimen holder Illumination housing Lamp Housing Phase contrast slider Microscope body Illumination intensity control knob Main Switch Eyepieces TCM 400 ERGONOMIC 7 TCM 400 5 7125000 795 TCM 400 Issue 1 3 Printed on April 2022 ...

Page 11: ...mponents before discarding packing material a Microscope body b Observation tubes as ordered c Eyepieces d Illumination housing e Stage extension plates f Specimen plate g Phase slider h Phase telescope i Objectives as ordered j Blue filter k Green Filter l Frosted filter m Installation tools n Power cord o Spare fuses as ordered p Spare bulb as ordered q Adapter for telescope r Dust cover Issue 1...

Page 12: ...r microscope from its packaging make sure that all of the following contents are present Please note that the contents of your microscope may vary as the optional configuration contrasting method or viewing body opted for may not be of the standard configuration highlighted here 9 TCM 400 STANDARD COMPONENTS 7 7125000 795 TCM 400 Issue 1 3 Printed on April 2022 ...

Page 13: ...8 INSTALLATION AND OPERATION OF OPTIONAL ACCESSORIES CAMERA MODULE SYSTEM 1 7125000 795 TCM 400 CMB CAMERA ADAPTER Blue filter Green filter Frosted filter Halogen Bulb Mechanical stage LW PH 4x LW PH 10x LW PH 20x LW PH 40x SL OPTIONAL WF 10x WF 16x WF 20x Binocular head Trinocular head Ergonomic head ATLAS CMOS CAMERA VEGA CMOS CAMERA iVU TOUCH SCREEN Issue 1 3 Printed on April 2022 ...

Page 14: ...e provided To replace 1 Pull out the 10x eyepieces out from the observation heads Ocular tube 2 Insert desired eyepieces in empty ocular tube Fig 3 1 11 TCM 400 OPTIONAL EYEPIECES 2 7125000 795 TCM 400 Issue 1 3 Printed on April 2022 ...

Page 15: ...d positions with the dust prevention caps to prevent from dirt and dust getting inside Install the observation head using the following procedure 1 Using allen wrench 3mm provided loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head i...

Page 16: ...ial focus mechanism turret stage plate and the lamp house arm as shown in figure fig 7 as shown 1 Insert the illuminator into the arm opening and rotate the illuminator 90 clockwise so that the letters NA 0 30 faces directly in front See fig 8 1 2 Tighten the clamping screw using a allen wrench 3 0mm provided with microscope See fig 8 2 Issue 1 3 Printed on April 2022 ...

Page 17: ...lider 3 The Blue filter is placed in the filter holder cartridge The cartridge can be easily fitted in the lamp house Hold the cartridge with your index finger and thumb and slide it in See fig 10 1 Loosen the Head Locking screw using allen wrench 2 0mm provided with the microscope to such a position where it does not come out See fig 11 2 Place the observation head on the stand and engage the dov...

Page 18: ...cus Adjust Diopter setting to 0 on the right side eyepiece and while looking through the right eyepiece with your right eye turn only the diopter adjustment ring to focus on the speci men See figures 12 and 13 The stage extension plate can be mounted in the left or right side or both sides of the stage to expand the stage surface However the stage ectension plate and mechanical stage cannot be use...

Page 19: ...he Lamp socket and pull out the blown bulb using a soft cloth Gently insert the new bulb Do not squeeze or press the bulb too much which can cause damage to the bulb and hand Fit the lamp socket by aligning the guide pins with condenser guide holes Push the lamp socket gently into the transmitted light illuminator Fig 15 16 Issue 1 3 Printed on April 2022 ...

Page 20: ...age the objective to be used in the light path and bring the specimen in focus Adjust the observation tube and eyepieces Adjust the interpupillary distance Adjust the dioptric setting Adjust the brightness Engage the required filters Observe Specimen On Off TCM 400 17 10 SUMMARY OF BRIGHT FIELD OBSERVATION PROCEDURE 7125000 795 TCM 400 Issue 1 3 Printed on April 2022 ...

Page 21: ...ng high Flask remove the condenser front lens by rotating it anti clock wise to fit in the flask As shown fig 18 In case your microscope has mechanical stage mounted and you are using either glass slide or 90 well plate use appropriate holders Focusing Procedure Figure 1 9 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We recom mend ...

Page 22: ...t it can be quickly referred to in the future This is happen when multiple users work with the microscope Adjust the Diopter setting to 0 on the left side eyepiece While looking through the left eyepiece with your left eye turn the coarse and fine focus adjustment knobs to bring the specimen into focus Adjust the Diopter setting to 0 on the right side eyepiece and while looking through the right e...

Page 23: ... is not correctly engaged Make sure that the revolving nosepiece clicks properly into place The specimen is not correctly mounted on the stage Place it correctly on the stage 5 The bulb flickers and the Brightness is unstable Use a vessel with a good profile Irregularity characteristic The line voltage fluctuates Use a voltage stabilizer 6 The coarse adjustment knob is too Difficult to rotate Repl...

Page 24: ...ensions Weight 495 0mm L x 300 0mm W x 470 mm H 9 5 kg net 8 Electrical Halogen 6V 30W 100V 240V AC 50 60 Hz Lamp life Lamp Input CAUTION HIGH VOLTAGE FUSE REPLACEMENT WARNING HIGH VOLTAGE POWER INLET EARTH WARNING HIGH TEMPERATURE Upto 500 hours 9 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to ...

Page 25: ...Revision History Rev No Date of Release Change App By DCR 1 3 April 14 2022 DCR 06 22 Reviewed Up dated for typo error S Bal ...

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