65
Adjustment specimen
For initial microscope adjustment we recom-
mend you use a specimen that has both high
and low contrast areas.
It is easier to focus incident light fluorescence
specimens in transmitted light first.
Focusing the specimen
(For the DM IRB version, please read the section about the
operation of the E focus and objective nosepiece first. Here,
an example of manual operation is given for each case.)
Focus the specimen you want to examine. To do
this, the objective nosepiece should be lowered
first. The objective is turned into the light path by
rotating the black knurled knob on the nose-
piece. The objective should click audibly into
position.
Focus the specimen with the coarse and fine
drive, which changes the height of the objec-
tive nosepiece. The stage height remains un-
changed. The total vertical travel of the
nosepiece is 7 mm. In air, the focusing range
extends from 2 mm below the stage surface to
5 mm above it.
One drum interval of the fine focusing cor-
responds to about 2 mm of the objective nose-
piece.
Caution!
Please be careful with high objective magni-
fications when focusing or making x-y adjust-
ments!
When using objectives with a high magnification
and a short working distance (from 50x), the
specimen and the stage insert may be lifted and
tilted.
When scanning the specimen, the front lens of
the objective may knock against the edge of the
stage insert.
Lower the coarse and fine drive if possible when
turning the nosepiece and changing the ob-
jectives, in order to avoid contact between the
front lens and the stage insert.
n. b.
Caution with special objectives! Here there may
be contact between the stage insert and the
front lens the moment the objective is moved
over the edge of the inner hole of the stage
insert!
!
!
