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4.12 Examinations in incident light fluorescence
Select a filter cube to suit the excitation and
emission spectrum of the specimen and switch
into the light path with the reflector turret (76.1).
All polarisation-optic components, such as the
polariser, analyser and ICR prisms must be re-
moved from the light path.
If included in the configuration, turn the magnifi-
cation changer (78.3) into position 1x to ensure
maximum light intensity.
Open the aperture diaphragm (76.5) and the field
diaphragm (76.4) to improve intensity.
If using Hg or Xe gas discharge lamps, an addi-
tional increase in intensity can be achieved by
assembling the illumination telescope (booster)
(8.4) between the lamphousing and the lamp-
housing mount.
Always block the incident light path when you
are not actually looking through the microscope
to prevent specimens fading.
This can be done by diverting the light at the mir-
ror housing with a lever (8.1) or changing the
position of the filter cube in the turret.
n.b.:
Remember the risk of glare due to high light
intensity when switching from the fluo filter
cube to the brightfield reflector.