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9. Contrasting Methods for Leica DM6000 B
9.1.2 Phase Contrast
• Switch to the transmitted light axis (TL) by
pushing the
TL/IL
button.
• Select the
PH
contrast (phase contrast) method.
Do so by pressing the
PH
variable key.
Alternatively: Press the
CHANGE TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display of the LeicaScreen, menu page
‘Status’ indicates
PH
.
• Insert a transmitted light specimen.
• Select an appropriate objective.
Objectives that are suitable for phase
contrast are engraved with PH.
• Focus on the image with the turning knob on
the SmartMove or with the focus wheel and
adjust the illumination with the
INT
-keys.
Notes:
• The microscope automatically selects the
correct light ring in the condenser.
• When selecting the phase contrast method,
the aperture diaphragm is opened completely
and can not be adjusted.
9.1.3 Dark Field (TL)
• Switch to the transmitted light axis (TL) by
pushing the
TL/IL
button.
• Select the
DF
(dark field) contrast method.
Do so by pressing the
DF
variable key.
Alternatively: Press the
CHANGE TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display of the LeicaScreen, menu page
‘Status’ indicates
DF
.
The dark field ring (dark field stop) is set au-
tomatically.
• Insert a transmitted light specimen.
• Select an appropriate objective.
• Focus on the image with the turning knob on
the SmartMove or with the focus wheel and
adjust the illumination with the
INT
-keys.
Notes:
• The maximum objective aperture which may
be used for dark field is
0.75
. When an invalid
objective is selected, the corresponding
button on the LeicaScreen flashes.
• The microscope automatically selects the
correct light ring in the condenser.
• When selecting the dark field method, the
aperture diaphragm is opened completely and
can not be adjusted.
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