Leica DMi8 A, Installation Manual

Page 1: ...Installation Manual Leica Microsystems CMS GmbH Manual 11934095 Revision 1 0 from 2015 01 15 Leica DMi8 A Leica DMi8 C Leica DMi8 M ...

Page 2: ...le or in part by print photocopy microfilm or other method including electronic systems is not allowed with out express written permission from Leica Microsystems CMS GmbH The instructions contained in the following documentation reflect state of the art technology We have compiled the texts and illustrations as accurately as possible Still we are always grateful for comments and suggestions regar...

Page 3: ... fluorescence illuminator 19 5 6 Equipping the incident light reflector turret 20 5 7 Assembling the polarizer and analyzer 21 5 8 Assembling the transmitted light illuminator arm TL 22 5 8 1 Tiltable transmitted light illuminator arm 22 5 8 2 Fixed transmitted light illuminator arm 23 5 9 Assembling the condensers for the tiltable transmitted light illuminator arm 24 5 10 Assembling the transmitt...

Page 4: ... minor or moderate injury Caution In this manual additional safety notes are indicated with the triangle symbol shown here and have a gray back ground Caution The instrument and accessories can be damaged when operated incorrectly Caution This Installation Manual is an essential component of the product it must be read carefully before the product is assembled put into operation or used and must b...

Page 5: ...etic field Warning of UV radiation Wear safety goggles Warning of hazardous laser radiation take appropriate safety precautions Warning of hand injuries Instructions for disposing of the instrument its acces sories and consumables Connection to ground Explanatory note Item not contained in all equipment configurations Manufacturing date e g 11 2011 for November 2011 China RoHS 50 years EFUP Enviro...

Page 6: ...rations to the instrument or noncom pliant use shall void all rights to any warranty claims 2 2 Technical data Compact Leica CTR electronics box For indoor use only Supply voltage 100 240 VAC Frequency 50 60 Hz Power consumption max 150 VA Fuses 3 15 A slow blowing breaking capacity H 250 VAC Size 5x20 mm Ambient temperature 15 35 C Relative humidity 90 up to 30 C non condensing Protection class I...

Page 7: ...ity 90 up to 30 C non condensing Protection class I Overvoltage category II Pollution degree 2 Leica DMi8 A microscope automated variants For indoor use only Supply voltage 100 240 VAC Leica CTR Frequency 50 60 Hz Leica CTR Power consumption see Leica CTR Fuses see Leica CTR Ambient temperature 15 35 C Relative humidity 90 up to 30 C non condensing Protection class I Leica CTR Overvoltage category...

Page 8: ...gm 9 Polarizer slot 10 Analyzer slot 11 Cover for right side port 12 Cover for motorized incident light turret disk 3 Overview of Components 1 Objective turret with objectives 2 Tube 3 Eyepieces and eyepiece tubes 4 Specimen stage 5 LED lamp housing 6 Colour filter slot 5 6 1 8 7 10 4 2 3 9 12 11 8 ...

Page 9: ...3 Overview of Components Fig 2 Left side of the Leica DMi8 A 1 Motorized DIC objective prism disk 2 Cover plate not used 3 Service port 4 Left side port with camera 1 3 4 2 9 ...

Page 10: ...yepieces and eyepiece tubes 2 Tube 3 Top port with camera 4 Cover plate not used 5 Incident light turret disk 6 Cover for left side port 7 Manual DIC objective prism disk 8 LED lamp housing 9 Objective turret with objectives 10 Specimen stage 1 2 4 6 7 8 9 10 5 3 10 ...

Page 11: ...ght stop 1 3 4 2 5 6 Fig 5 Right side of the Leica DMi8 with tiltable transmitted light illuminator arm 1 Transmitted light filters 2 Transmitted light lamp housing receptacle 3 Condenser with motorized polarizer Fig 6 Left side of the Leica DMi8 with fixed transmitted light illumina tor arm 1 Stop lever for condenser height adjustment 2 Fixed transmitted light illuminator arm 3 Transmitted light ...

Page 12: ...t or its packaging have visible damage do not put the instrument into operation Note If at all possible avoid touching optical surfaces such as the lens surfaces on the objective If fingerprints do appear on the glass surfaces remove them with a soft leather or linen cloth Even small traces of finger perspiration can damage the surfaces in a short time See the chapter on Care of the Microscope for...

Page 13: ...awayfromthewallandawayfromflammablesubstances Weight The weight of the microscope depends on the respective equipment configuration CAUTION At a full load the microscope weighs more than 18 kg The user must take corresponding precautions for trans porting the instrument 13 ...

Page 14: ... lamp housing DIC module and DIC objective prisms Specimen stage Eyepieces Objectives Incident light illuminator LED lamp housing Equipment for the incident light turret Polarizer and analyzer When using other systems and advanced optical accesso ries the sequence may vary Only a few commonly used screwdrivers and keys are necessary for assembly these are included in the delivery package 5 Assembl...

Page 15: ... disk so that the electronics board is fac ing downwards Do not touch the electronics especially the contacts with your fingers Upgrading individual IC prisms Loosen both Allen screws and remove the prism disk Place the prisms against the peg Fig 7 3 and the shim between the prism and screw Fig 10 and lightly tighten to avoid tension Insert the prisms so that the identifying letter or ID is facing...

Page 16: ...ar holes Align the stage so that both holes point back towards the illumination axis and the single hole is facing in the direc tion of the tube Align the fastening holes in the stage with the holes in the contact surface If the holes are not visible as in the case of the 3 plated cross stage or scanning stage gen tly move the upper tabletop until the opening is visible Start by gently turning the...

Page 17: ...djustment are included with some not all inserts Finally affix these scales in the recesses of the mechani cal stage Motorized 3 plate or scanning stage 3 plate stages and scanning stages After assembling the stage first connect in the case of motorized stages the provided stage cable to the socket on the stage and then to the electronics box The corre sponding mark on the box is called XY Stage V...

Page 18: ...g 18 Depending on your equipment specific positions have already been assigned to the individual objectives at the factory For details on the exact positions of the objectives please refer to the enclosed Identification sheet Caution Close vacant threads in the turret using dust protection caps Please note that the front lenses of the objectives face up wards This subjects them to increased exposu...

Page 19: ... with motorized incident light method Connect the cable of the lamp housing to the power sup ply for transmitted light for the Leica CTR electronics box Lamp RL port 1 Fig 20 Fastening the lamp housing to the incident light axis 1 Fastening screw 5 5 2 Assembling the fluorescence illuminator Leica EL6000 See separate manual CAUTION When using the Leica EL6000 compact light source it is essential t...

Page 20: ...isk Fig 22 1 The cover is secured in place by a magnetic holder and can be detached from the front With the holder facing you squarely slide the filter cube or reflector cube forwards into the holder as described in the identification sheet provided Check to ensure that the cube is placed properly A loose cube can be damaged when rotating the turret disk or can block the disk Once all filter block...

Page 21: ...the incident light turret disk Fig 25 1 Insert the polarizer into the receptacle until it latches in place Fig 25 1 Manual incident light analyzer Remove the plug cap on the right side of the stand below the incident light turret disk Fig 25 2 Insert the analyzer into the receptacle until it latches in place Fig 25 2 Fig 25 1 Incident light polarizer 2 Incident light analyzer 1 2 21 ...

Page 22: ...smitted light il luminator arm This ensures optimum alignment for the optical axis The tilting angle of the illuminator arm can be modified or locked in place in the vertical position using the knurled screw Fig 27 2 Leica DMi8 A Connect the electronics cable to one of the EXT1 EXT6 sockets Fig 27 3 Fig 26 Transmitted light illuminator arm assembly 1 Transmitted light illuminator arm 2 Pin on tran...

Page 23: ...er Also see the following note The marks Fig 29 6 pertain to a liquid height of 15 mm For stands with double marks the upper and lower lines represent a range with different liquid heights Make sure that the transmitted light illuminator arm is engaged Connect the electronics cable to the port Fig 30 3 Note For the stands with 3 plate mechanical stages position the illuminator arm 25 mm below the ...

Page 24: ...k and can be individually equipped with the cor responding ring diaphragms for phase contrast PH dark field DF or IC prisms for transmitted light differential inter ference contrast DIC Usually the light rings and condenser prisms have already been inserted into the turret disk at the factory eliminating the need for subsequent assembly For more information read Page 28 Assembling condensers Fig 3...

Page 25: ...tercept of the corresponding condenser head and DF diaphragm marked with D for dark field and the S focal intercept of the corresponding condenser head into the receptacle drill holes of the turret disk as follows Select an opening and check to ensure that both fasten ing screws are loose enough so that they are no longer protruding into the opening Move the desired light ring opening into the bea...

Page 26: ...8 Caution Never press down on the spring clip This can destroy the clip or put the light ring in an unstable position Make sure that the light ring clicks into place through ro tation and loosen the clamp If necessary carefully remove fingerprints or dust from the prism The light ring is precentered using the left centering screw The right centering screw must never limit the range of movement Wri...

Page 27: ...mp The prism can be grFiged by pressing down on the side prongs of the clamp Fig 38 Two guiding ridges can be found on the bottom of the prisms These ridges must fit into the two grooves in the opening The prism must be inserted condenser clamp from above at a slight angle and at an angle of approx 90 to the housing so that the mount is grips under the spring clip of the holder Fig 38 Caution Neve...

Page 28: ...lace the condenser on top of the locking pins of the transmitted light illuminator arm and then move the con denser to the corresponding height Marks on the column and the condenser help you in finding the correct dis tance Tighten the Allen screw once you have reached the cor rect position Fig 39 Assembling the condenser on the transmitted light illuminator arm Fig 40 Condenser mounted on transmi...

Page 29: ...olarizer Preconfigured at the factory Proceed as follows when performing upgrades Motorized condenser See the enclosed Assembly manual Manual condenser Fasten the 1 position or 3 position position holder onto the top of the manual condenser as necessary The holder has a guide that must be inserted into the opening next to the screw thread The holder must be positioned so that the polarizer to be i...

Page 30: ...ht lamp housing receptacle Fig 45 and fasten it with the side clamping screw Route the cable into the cable shaft on the rear side of the transmitted light illuminator arm Fig 46 Leica DMi8 M and C with manual transmitted light method Connect the cable of the lamp housing to the port Fig 47 1 on the rear side of the microscope Leica DMi8 A with motorized transmitted light method Connect the cable ...

Page 31: ...Socket for power cable Leica DMi8 A Connect the incident light illuminator port Fig 49 3 of the rear side of the microscope to the Lamp RL port Fig 50 Fig 51 1 on the electronics box using the lamp cable Connect the Microscope socket Fig 50 Fig 51 4 to the rear side of the stand Fig 49 1 Use the 25 pin micro scope cable for this If present connect the Leica SmartMove control element or Leica STP40...

Page 32: ...d light il luminator arm to one of the EXT1 EXT6 sockets Fig 49 2 Connect the lamp cable of the transmitted light illumi nator arm to the Lamp TL port Fig 50 Fig 51 2 on the electronics box Fig 50 Rear side of compact Leica CTR electronics box 1 Connection for lamp cable for incident light 2 Connection for lamp cable for transmitted light 3 2 x USB 4 Microscope connection 5 Socket for power cable ...

Page 33: ...the light field diaphragm until it just disappears from the field of view This setting of the field diaphragm remains the same for all objectives It is necessary to readjust the field diaphragm only when using eyepieces with different field numbers or when changing the magnification using a magnification chang er and for photography and TV imaging Narrowing the field diaphragm normally improves th...

Page 34: ...suitable for phase con trast the corresponding light ring is introduced automati cally motorized condenser or you must manually swing the corresponding light ring inwards Leica DMi8 with manual condenser If necessary set the TL bright field position or empty po sition on the fluo turret or select the DAPI TL filter cube Select the bright field position on the condenser Leica DMi8 with motorized co...

Page 35: ...rrower bright ring light ring in condenser Fig 55c Caution When changing the objective the centering key must no longer be in the corresponding openings You can lock the condenser Repeat the process for all other phase contrast objec tives Always remove the centering keys after the centering procedure Note You can find more information on the phase contrast meth od in Leica Science Lab Link http w...

Page 36: ...DMi8 with motorized condenser and polarizer Select the POL method If the analyzer is present on the fluo turret as an analyzer block it will automatically swing inwards A manual analyzer must be manually inserted On motorized condensers with motorized polarizers the polarizers move into position automatically Insert the centering key into the corresponding openings on the condenser Fig 57 Set the ...

Page 37: ...ld diaphragm until the image fills almost the entire im age field The black edges of the image should have the same distance from the outer edge of the field of view all the way around If this is not the case center the image again using the centering screws Use the condenser height adjustment to bring the edges into focus Open the field diaphragm until the image fills the entire field of view and...

Page 38: ...IC prisms 15 Identification sheet 18 20 Illuminator 19 Incident light reflector turret 20 Incident light turret disk 8 10 Inserts 17 Installation location 12 K Koehler illumination 37 L Lamp cable connection 31 LAS software 18 26 LED lamp housing 10 19 Leica CTR electronics box 31 32 Leica EL6000 19 Leica Science Lab 35 37 Light guide 19 Light ring 25 26 35 Light ring slide 11 Light source 19 M Me...

Page 39: ......

Page 40: ...Leitz Strasse 35578 Wetzlar Germany 2015 Phone 06441 29 0 Fax 06441 29 2599 LEICA and the Leica logos are registered trademarks of Leica IR GmbH Order Nos of the editions in English 11934095 Printed on chlorine free bleached paper III 15 M H Revision 1 0 issued 2015 01 15 ...