15
Appendix
Adding a scale bar to an image
1. On an open image select the Scale icon from the set of tools left of the
displayed image
2. Hold the left mouse button and drag across the image to draw a scale
bar
3. To edit the length or position of the bar select the Cursor icon then click on one
end of the scale bar to adjust its length or in the middle of the scale bar to move
it within the image
4. To delete the scale bar select the bar using the cursor tool then select the Delete
button
5. To save a copy of the image with the scale bar, right click on the
image then select “Snapshot all”
Exporting Images
1. To export an experiment or single image, right click on it in the Experiments view
then select Export>As TIFF.
2. Select the destination folder for the exported images.
3. The Overlay Channels option will save a single file
(RGB colour) that contains all of the wavelength
channels captured. This is not the raw data and
cannot be used for quantitative intensity analysis.
4. If the Overlay Channels option is not ticked then a
Raw data check box appears, select this option. The
greyscale 16bit images will be saved as individual
TIFF files.
Summary of Contents for SP5 confocal
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