5
Mounting a sample
1.
Ensure the correct stage insert is mounted (see facility staff if
unsure)
2.
Remember to invert your slide so that the cover glass is the closest
surface to the lens
3.
Select the objective lens either using the two buttons behind the right focus
wheel or in the software within the Acquire tab then select the Objective text to
see a drop down list of lenses
4.
Use the joystick to move the stage if necessary. Change the XY speed
of the stage by pressing the black buttons on the left side of the joystick
base. The black buttons on the right change the speed in Z.
Finding the sample by eye
1. Choose a fluorescence filter cube via the buttons on the front of the microscope.
Push the shutter button to toggle open/close shutter.
I3 = FITC
N2.1 = TxRd
A = DAPI
2. Once the sample is in focus you can record this Z
position by holding the two outer Set Z buttons on the
right side of the microscope. This sets this Z position to
X
Y
Z
Fluorescence
shutter
Filters
Focus speed
(course/fine)
Observation
mode BF/FL
BF intensity
(Controller on
LHS of microscope
Z position
Summary of Contents for SP5 confocal
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