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Rolly Wiegand – CALM Leica SP5 manual
17
The image acquisition in transmitted light mode requires the use of the 488 nm Argon laser
line and can be recorded simultaneously with the fluorescence channel (the laser line
activated and both detectors switched on). The settings of the condenser are being
checked and adjusted frequently and thus should be correct. However, if you feel that it
needs to be re-adjusted and if you are not familiar with the condenser unit, please inform
any CALM staff member.
Fig. 22
Both channels with correct settings
4.4.7. Saving and loading of Beam Path settings
Once you have finished adjusting all the settings, these can be saved to the hard disk for
later re-use. In the ‘Beam Path Settings’ screen click ‘save’ (Fig. 24 A). The system then
will prompt you for a name and a file. Please generate a unique file name you can
recognise later on and save the file in your folder on disk D:. This function saves the
activated laser lines, laser power settings, slit positions and PMT settings.
Fig. 23
Setting of the transmitted light channel
A
B
C
