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Rolly Wiegand – CALM Leica SP5 manual
1
1. Starting up the SP5
1.1. Before you switch on the system, fill in the log book (start time, lasers used, hours on
mercury lamp power supply, etc).
1.2. Switch on all three switches on the TCS control panel in the order shown in Fig. 1
(PC/Microscope, Scanner Power and Laser Power) and then turn the key 90
°
clockwise
(Laser Emission).
Fig. 1
1.3. Wait until the PC has finished booting up.
1.4. Log into Windows XP using the general account ‘TCS user’, which is not password
protected.
1.5.
IMPORTANT
: Make sure that the objective turret on the microscope stand is set to its
lowest position and that the condenser is in its normal position well clear of the stage. The
fastest method to do this is to press the ‘Z
↓
’ button on the right hand side of the
microscope base (see Fig. 6).
1.6. Start the LAS AF software by double clicking the icon on the desktop. The following
window will appear (see Fig. 2A). Choose ‘ok’ without going into the configuration or
microscope stand setup.
Fig. 2A
