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Rolly Wiegand – CALM Leica SP5 manual
6
Fig. 7
Front panel of the microscope stand
2.9. To focus on your sample, you can use the two main knobs on both sides of the
microscope stand (see Fig. 6). The focus operates in ‘coarse’ and ‘fine’ mode and you can
switch between the two modes by pressing the upper button on the right hand side
SmartMove remote control (see arrow in Fig. 8). To mark a desired Z-position of the focus,
press the two buttons ‘Z
↑
’ and ‘SET’ simultaneously (see Fig. 9). Do not use the ‘Z
↓
’
button. If you simultaneously press the buttons the first time, this will delete any previous
setting and the display at the front of the stand should display ‘--,--
μ
m’. If you press them
again, the display will be changing to ‘0
μ
m’ and this is your reference setting. The display
will give you a constant read-out f the actual Z-position (see Fig. 10) and allows you to
conveniently find your initial, marked focal plane again.
IMPORTANT
: Never push the objective lens against the coverslip, because this might
damage the front lens and might require a very expensive repair.
2.10. To quickly move the objective turret to the lowest point, e.g. for making sure that the
lens is out of the way for stage initialisation or after finishing with your work, press the ‘Z
↓
’
button (see Fig. 9).
2.11. The SmartMove remote control allows you to conveniently control several functions
of the microscope such as the X- and Y-movement of the motorised stage and the Z-drive
mode (see Fig. 8).
3. Rules for the use of the mercury vapour arc lamp
3.1. Only turn the lamp (ebq 100, see Fig. 5) on when it is cool, i.e. when it has been
switched off for 30 minutes or longer. The lamp must not be switched on when it is still
warm from the previous use. Check the system log sheet for information about when the
lamp was turned off the last time. If in doubt, contact the previous user.
3.2. Once the lamp has been switched on, it has to be left on for a minimum of 30 minutes.
A
N2.1
I3
