To view the specimen in bright field (BF), 4 things to do: turn the power wheel # 11 on to
about 4V
(for white light at 3200 K around 8V, to be used with NDF)
and the lollypop
shutter above the field diaphragm in down position
,
Knob #12 operating a mirror in the
condenser light path should be on VIS = visual and MicCtrl on Visual.
Focus on the specimen with the knob “Focus” ; step S3 is coarse, step S2 is medium, Step
S1 is fine.
Apply Koehler illumination by closing diaphragm #13a and adjusting the level of the
condenser at knob # 13b to obtain a sharp projection of the edges of this diaphragm in the
oculars. The illumination path can be centered with the two rings #13c.
Set #15 on BF. If more suitable for a certain objective 63x oil or 40/63 x).
Knob #22 should be pushed inward
Adjust diaphragm #14 (from 0 to over 8 and Ph) to have just enough contrast.
Light can be dimmed with Neutral density filter (NDF) # 16. For bright field illumination
pull polarization filter # 17 out of the light path.
Turn the ribbed disc # 18 to position BF.
Pull the (ICT P) analyzer (polarized filter) #19 out for bright field vision
Keep #22 pressed in (half pulled out is 50% ocular-50% upper camera port vision; fully
pulled out is 100% upper camera port vision).
Ring #23a (display #23b) can be set in position 1x (open connection for bright field); UV
for ultraviolet scan stand, B for phase contrast adjustment with Bertrand lens (not relevant
in this microscope) , and scan for non-UV scanning
Optional but easy is the function to set the z-focus level of the objective for later recovery.
Press #27 ↑ for 3 s, focus and press #27 ↑ again. The lowest position of the objectives can
be set in a similar way using the #25↓ switch (standard is around –150 micrometer).
Differential Interference contrast settings
: Press the (ICT P) analyzer polarized filter
#19 in for eye vision and out for laser scanning for this S23 condenser, turn either of the
following prism on the #15 turret to the front: K10 for 63x gly and oil objectives, K8 for
20 x and D1 for 10x. Switch polarizer/analyzer #16 into the light path.
To watch wide-field fluorescence, choose the desired filter block with the arrows on 20d,
and open shutter 20c (close the transmission light lolly pop)
To go to the confocal imaging, choose scan from the MicCtrl icon, block the light of the
transmission lamp, close the fluorescence lamp shutter.
