B. Acquiring and saving images
In the Leica Confocal Software (icon on desktop): the "
Acquire
" menu appears on the left
monitor. Images will be displayed on the right
monitor.
Switch on appropriate lasers and push the
control for each wavelength to the desired level
in %. Click active. For the 405 nm laser choose
% passed through Neutral density filter (--
means no neutral density filter).
Set PMTs for the desired wavelength ranges by
mouse dragging (trans active optional;
reflection is a special case). Functions of the
black knobs can be adjusted by right clicking
on the lower bar menu (choose configuration
for menu, choose sensitivity to adjust the coarsness of the knob): e.g. Smart offset, smart
gain. Use these knobs to control the offset and gain levels, pinhole opening or zoom factor
or to focus.
