Leica TCS SP5 X, User Manual

Page 1: ...Leica TCS SP5 Leica TCS SP5 X User Manual ...

Page 2: ...ems CMS GmbH Am Friedensplatz 3 D 68165 Mannheim Germany http www leica microsystems com http www confocal microscopy com Responsible for contents Leica Microsystems CMS GmbH Copyright Leica Microsystems CMS GmbH All rights reserved ...

Page 3: ...16 2 7 2 Electrical Connection Requirements 17 2 7 2 1 Electrical connection requirements of supply unit 17 2 7 2 2 Electrical connection requirements of achromatic light laser 17 2 7 3 Requirements Regarding Ambient Conditions 18 2 7 4 Permitted Ambient Conditions 18 2 7 5 Waste Heat Required Cooling Performance 19 2 8 Features 19 2 8 1 Overview of Usable VIS UV Lasers 19 2 8 2 Overview of Usable...

Page 4: ...interlock jack interlock connector on the scanner 37 5 8 Function and Position of Safety Switches 38 5 9 Special Laser Safety Equipment 39 5 9 1 Laser protection tube and beam stop 39 5 9 2 Shielding in MP Systems IR Lasers 40 5 10 Safety labels on the system 41 5 10 1 Inverted microscope DMI 6000 CS 41 5 10 2 Upright microscope DM 5000 6000 CS 43 5 10 3 Scan Head 45 5 10 4 Achromatic light laser ...

Page 5: ...ing Up Users 72 8 Switching Off the System 73 9 Introduction to LAS AF 75 9 1 General 75 9 2 Online Help 75 9 2 1 Structure of the Online Help 75 9 2 2 Accessing the Online Help 76 9 2 3 Full text Search with Logically Connected Search Terms 76 9 3 Structure of the graphical user interface 78 9 3 1 General Structure of the Graphical User Interface 78 9 4 Key Combinations 79 10 Introduction to Conf...

Page 6: ...ion Thicknesses 106 10 4 3 Distances 107 10 4 4 Data Volumes 107 10 4 5 Depictions 108 10 4 5 1 Gallery 108 10 4 5 2 Movie 108 10 4 5 3 Orthogonal Projections 109 10 4 5 4 Rotated Projections 110 10 5 Time Series 110 10 5 1 Scan Speed 110 10 5 2 Points 111 10 5 3 Lines 111 10 5 4 Planes 111 10 5 5 Spaces Time Space 111 10 5 6 FRAP Measurements 112 10 6 Spectral Series 112 10 6 1 Data Acquisition a...

Page 7: ... System 116 12 Transport and Disposal 117 12 1 Changing the Installation Location 117 12 2 Disposal 117 13 Contact 117 14 Glossary 118 15 Appendix 124 15 1 Safety Data Sheets from Third party Manufacturers 124 15 2 Declaration of conformity 129 15 3 People s Republic of China 130 ...

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Page 9: ...r in part by print photocopy microfilm or other method including electronic systems is not allowed without express written permission from Leica Microsystems CMS GmbH Programs such as LAS and LAS AF are protected by copyright laws All rights reserved Reproduction adaptation or translation of these programs is prohibited without prior written permission from Leica Microsystems CMS GmbH The term Win...

Page 10: ...ctivating and commissioning the system and provides important information about the Leica Application Suite Advanced Fluorescence LAS AF software The system is delivered with the latest version of the licensed LAS AF To maintain information on the most current level the description of software functions was intentionally omitted from this operating manual Instead reference is made to the online he...

Page 11: ...11 2 Leica TCS SP5 TCS SP5 X 2 1 TCS SP5 system overview Figure 1 TCS SP5 system components overview 1 TCS SP5 Scanner 2 Main switch board 3 TCS workstation 4 Supply unit 5 Control panel 6 Microscope ...

Page 12: ...omatic light laser 2 3 Intended Use The system was designed for confocal scanning laser scanning images of fluorescence marked living and fixed specimens as well as for quantitative measurements in the area of life science This system is intended for use in a lab Applications of in vitro diagnostics in accordance with MPG German Medical Devices Act are excluded from proper intended use ...

Page 13: ...A This is a Class A instrument for use in buildings that do not include domestic premises and buildings not directly connected to a low voltage power supply network that supplies buildings used for domestic purposes IEC EN 61000 3 2 Electromagnetic Compatibility EMC Part 3 2 Limits Limits for harmonic currents IEC EN 61000 3 3 Electromagnetic Compatibility EMC Part 3 3 Limits Limitation of voltage...

Page 14: ... the US patents 5 886 784 5 903 688 6 137 627 6 222 961 6 285 019 6 311 574 6 355 919 6 423 960 6 433 814 6 444 971 6 466 381 6 510 001 6 567 164 6 611 643 6 614 526 6 654 165 6 654 166 6 657 187 6 678 443 6 687 035 6 710 918 6 738 190 6 754 003 6 796 699 6 801 359 6 806 953 6 831 780 6 850 358 6 867 899 6 888 674 6 898 367 6 958 858 6 961 124 7 005 654 7 092 086 7 110 645 7 123 408 Further patent...

Page 15: ...P5 with inverted microscope 1400 mm 600 mm 730 mm 1200 mm 2540 mm Figure 4 Dimensions of TCS SP5 with inverted microscope 2 7 1 2 TCS SP5 with upright microscope 600 mm 730 mm 2400 mm 1490 mm 1200 mm Figure 5 Dimensions of TCS SP5 with upright microscope ...

Page 16: ...16 2 7 1 3 TCS SP5 X with inverted microscope Figure 6 Dimensions of TCS SP5 X with inverted microscope 2 7 1 4 TCS SP5 X with upright microscope Figure 7 Dimensions of TCS SP5 X with upright microscope ...

Page 17: ...lasers such as UV and MP lasers please refer to the manufacturer s documentation 2 7 2 1 Electrical connection requirements of supply unit Supply voltage 100 240 V AC 10 Frequency 50 60 Hz Power consumption 3200 VA Overvoltage category II 2 7 2 2 Electrical connection requirements of achromatic light laser 1 Supply voltage 100 240 V AC 10 Frequency 50 60 Hz Power consumption 2 2 A Overvoltage cate...

Page 18: ...ened rooms is also advisable The system requires doors with inside widths of 1 0 m for installation maintenance and transport With regard to the load bearing capacity of the floor note that the system will apply a static load of 200 kg m Ensure that the environment is as vibration free as possible 2 7 4 Permitted Ambient Conditions Permissible temperature range for operation 18 to 25 ºC Temperatur...

Page 19: ...e lasers listed below Laser type Wavelength nm Maximum luminous power at laser output mW Maximum luminous power in focal plane mW Pulse duration Diode 405 405 120 7 Continuous wave cw Diode 405 p 405 5 mean power 0 3 mean power pulsed 60 ps DPSS 445 445 75 7 Continuous wave cw Ar 458 476 488 496 514 200 50 Continuous wave cw Ar external 458 476 488 496 514 500 125 Continuous wave cw HeNe 543 1 5 0...

Page 20: ...nous power at laser output W Luminous power in focal plane W Pulse duration MaiTai ps 780 920 1 2 0 6 pulsed 1 0 1 5 ps MaiTai ps wideband 710 950 2 5 1 2 pulsed 1 0 1 5 ps MaiTai ps broadband 710 990 2 5 1 2 pulsed 1 0 1 5 ps MaiTai ps HP 690 1040 3 0 1 9 pulsed 1 0 1 5 ps Chameleon ps Ultra 690 1020 4 1 9 pulsed 1 0 1 5 ps Chameleon ps Ultra I 690 1040 4 1 9 pulsed 1 0 1 5 ps Chameleon ps Ultra ...

Page 21: ...MaiTai HP Deep See 690 1040 3 0 1 9 pulsed 100 fs Chameleon fs Ultra 690 1020 4 1 9 pulsed 140 fs Chameleon fs Ultra I 690 1040 4 1 9 pulsed 140 fs Chameleon fs Ultra II 690 1080 4 1 9 pulsed 140 fs Chameleon Vision I 690 1040 4 0 1 9 pulsed 140 fs Chameleon Vision II 680 1080 4 0 1 9 pulsed 140 fs 680 1080 4 0 1 9 pulsed 140 fs Chameleon Compact OPO 1000 1600 1 6 0 8 pulsed 100 fs Table 3 Usable ...

Page 22: ...l plane mW Pulse duration Diode 405 405 120 7 Continuous wave cw Diode 405 p 405 5 mean power 0 3 mean power pulsed 60 ps UV external 355 500 18 Continuous wave cw Ar 458 476 488 496 514 200 50 Continuous wave cw 488 solid state laser 488 470 70 Continuous wave cw Achromatic light laser 470 670 500 50 Pulsed Table 4 Usable lasers for TCS SP5 X The components for laser safety are designed only for ...

Page 23: ... lasers from Chapter 2 8 3 or 2 8 1 VIS and UV lasers 3B IIIb MP 350 1600 nm visible and invisible laser radiation Combination of lasers from chapter 2 8 1 VIS UV Laser chapter 2 8 2 IR Laser or chapter C2 8 3 VIS UV Laser 4 IV 2 8 5 Required Laser Safety Measures Please observe the laser safety measures for laser class 3B IIIb VIS and UV systems or laser class 4 IV MP systems in accordance with a...

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Page 25: ...HARMFUL SUBSTANCES A harmful substances warning points out a substance that can be harmful to your health CAUTION A safety instruction points out an operation a process a condition or an instruction that must be observed strictly to prevent severe damage to the system or loss of data WEARING PROTECTIVE EYEWEAR This mandatory sign draws your attention to the fact that suitable eye protection gear m...

Page 26: ...26 Notes either contain additional information on a specific topic or special instructions on the handling of the product ...

Page 27: ...oes the owner operator have to observe The owner operator of this product is responsible for proper and safe operation and safe maintenance of the system and for following all applicable safety regulations The owner operator is fully liable for all consequences resulting from the use of the system for any purposes other than those listed in the operating manual or the online help This laser produc...

Page 28: ...l The owner operator is fully liable for all consequences resulting from the use of the system if it is opened improperly serviced or repaired by persons other than authorized Leica service representatives If repairs or service measures are performed that require opening parts of the housing only trained Leica service technicians may occupy the room in which the system is located Do not connect an...

Page 29: ...tion in the online help For an overview of the individual functions refer to the table of contents of the online help As it is impossible to anticipate every potential hazard please be careful and apply common sense when operating this product Observe all safety precautions relevant to Class 3B IIIb lasers and Class 4 IV lasers for MP systems Do not deviate from the operating and maintenance instr...

Page 30: ...the Supply Unit The total power consumption of all loads connected to the multiple socket outlet Figure 8 must not exceed 800 VA The terminals are intended for TCS workstation Monitor 1 Monitor 2 Microscope Figure 8 Multiple socket outlet rear side of supply unit ...

Page 31: ...e the complete system using a single switch Figure 9 The main circuit breaker functions as a switch and as an overcurrent fuse The main circuit breaker is not to be used as the regular on off switch for the system The supply unit must be set up so that the main circuit breaker is freely accessible at all times Figure 9 Supply unit with main circuit breaker ...

Page 32: ...on the main switch board see Figure 10 Figure 10 Detachable key switch for the internal lasers The detachable key switch for protection against unauthorized use of the external achromatic light laser is located on the front of the achromatic light laser see Figure 11 Figure 11 Detachable key switch for the achromatic light laser ...

Page 33: ...xternal UV laser is located on the front of the power supply see Figure 12 Figure 12 Key switch for the external UV laser For other external lasers please refer to the operating manual supplied by the laser manufacturer for the position of the detachable key switches ...

Page 34: ...f the achromatic light laser is located on the front of the achromatic light laser see Figure 14 and is red when lit As soon as the emission warning indicator of the lasers is lit it is possible from a functional standpoint that laser radiation is present in the specimen area Figure 13 Emission warning indicators on the main switch board Figure 14 Emission warning indicator at the achromatic light...

Page 35: ...acturer for the position of the emission warning indicator 5 4 Remote Interlock Connection on the Supply Unit The remote interlock jack is located on the rear side of the supply unit 12 V DC operating voltage Figure 16 The remote interlock plug which contains a shorting bridge is connected to this jack Remote interlock devices such as those connected to the room the door or other onsite safety int...

Page 36: ...connected to the remote interlock jack of the white light laser If you operate the white light laser separately without connecting it to the TCS SP5 system you have to use the remote interlock jack on the white light laser see Figure 17 for connecting remote interlocks Remote interlock devices such as those connected to the room the door or other onsite safety interlock systems can also be connect...

Page 37: ...lock jack interlock connector on the scanner The interlock jack is located on the rear side of the scanner operating voltage 12 V DC Figure 18 For laser safety reasons the inverted microscope must be connected to this connection or if an upright microscope is used the mirror housing This ensures that the safety switch of the microscope is integrated in the interlock circuit Figure 18 Location of t...

Page 38: ...Type of microscope Activated if Function 1 Transmitted light illuminator arm Inverted microscope DMI 6000 CS The illuminator arm is tilted e g for working on the specimen Prevents laser light while working on the specimen 2 Motorized changeover between scanning mode and eyepiece Inverted microscope DMI 6000 CS The deflection mirror for the scanner is swung out by motor Prevents stray light if the ...

Page 39: ... a condenser base Figure 20 item 3 be aware that the condenser base is delivered without the beam stop Figure 20 item 2 The existing beam stop Figure 20 item 2 must always be reinstalled Please consult the microscope s operating manual provided When using a condenser base with filter holder always make sure that unused filter holders are swung out of the beam path and that the laser protection tub...

Page 40: ... and therefore completely shielded until it leaves the microscope objective and reaches the specimen For systems with infrared laser wavelength range 700 nm the beam is passed through a safety beam guide and if necessary also passed through a fiber optic cable Figure 21 This shields the laser beam until it leaves the microscope objective and reaches the specimen Figure 21 Safety beam guide 1 and I...

Page 41: ...ected dependent on the laser configuration VIS UV MP and attached in the following locations either in the English or German language 5 10 1 Inverted microscope DMI 6000 CS Angled rear view of right side of microscope Figure 22 Safety label for inverted microscope DMI 6000 CS ...

Page 42: ...42 Angled front view of right side of microscope Figure 23 Safety label for inverted microscope DMI 6000 CS ...

Page 43: ...43 5 10 2 Upright microscope DM 5000 6000 CS Angled front view of right side of microscope Figure 24 Safety label for upright microscope DM 5000 6000 CS ...

Page 44: ...44 Rear view of microscope Figure 25 Safety label for upright microscope DM 5000 6000 CS ...

Page 45: ...45 5 10 3 Scan Head Angled front view of left side of scan head Figure 26 Safety label for the scanner ...

Page 46: ...46 5 10 4 Achromatic light laser 3 Rear side of achromatic light laser Figure 27 Safety label on the rear side of the white light laser 3 Applies only to the TCS SP5 X system ...

Page 47: ...47 5 10 5 External UV laser4 Figure 28 Safety label on external UV laser 4 Applies only to systems with an external UV laser ...

Page 48: ...48 5 10 6 Supply Unit View of supply unit Figure 29 Safety label for the TCS SP 5 supply unit front side ...

Page 49: ...49 5 10 7 MP beam coupling unit Angled front view of the right side of the MP beam coupling unit Figure 30 Safety label for the MP beam coupling unit top side ...

Page 50: ...ge Front view of cover Figure 31 Cover for replacement flange If the replacement flange for transmitted light is not equipped with a functional module such as a lamp housing a cover must be placed over the opening for laser safety reasons ...