6
•
Rotate the focusing knob to slowly raise the stage until the objective is close to the
specimen; keep checking the distance between the objective and the object to avoid their
contact. CAUTION: The objective should not touch the specimen, otherwise the objective or/
and the specimen might be damaged.
•
Look through the installed eyepiece and lower the stage slowly rotating the focusing knob
until you see the sharp image of the observed specimen.
•
Such adjustment protects the frontal lens from contacting the object when you use
objectives of other magnifications; though, slight refocusing might be required.
•
If the image is too bright, adjust the diaphragm disk until the passing light ray is reduced to a
comfortable brightness level. If the image is too dark, increase the light ray.
Selecting the objective
•
Start your observations with the lowest magnification objective (4x) and select a specimen
segment for detailed research. Then move the specimen to center the selected segment in
the field of view, to make sure it keeps centered when the objective is changed to a more
powerful one. Once the segment is selected, you should center its image in the microscope’s
field of view as precisely as possible. Otherwise, the desired segment might fail to center in
the field of view of the higher power objective.
•
Now you can switch to a more powerful objective by rotating the revolving nosepiece. Adjust
the image focus, the iris diaphragm and the illumination brightness if required.
•
When using a 100x objective lens, you have to fill the space between the lens and the
specimen with immersion oil. There should be no air bubbles in the oil, as they might reduce
the resulting quality of the image. Do not use any substitutes for the immersion oil, as
this will significantly reduce the quality of the resulting image
. When you are finished
observing, clean the immersion oil with a cloth or filter paper. If the objective lens is stained,
you can clean it with a cotton pad dipped in isopropyl alcohol. Do not apply any excessive
pressure, as you may damage the objective lens!
Digital camera
Levenhuk D320L BASE and D320L PLUS come with a digital camera. The digital camera is installed
in the eyepiece tube in place of the eyepiece. It allows you to observe specimens in fine detail and
true colors on your PC monitor and save images on the hard drive. Connect the camera to your PC
via a USB-cable that also acts as a power cable.
