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Chapter 2: Pearl Clean Box

3) Turn on the docking station and wait for the heater plate to reach the temperature set point

(green “running” icon is ON constantly).

Note:

Alternatively, the heater plate can be warmed by locking the Clean Box in the Pearl ima-

ging drawer until the set point temperature is reached (see the Pearl Imager Operator's Manual).

4) Make sure the nose cone plug is in place and start the flow of anesthesia gas as described in

Docking Station Operation

on page 6. Observe all cautions for the use of isoflurane gas. Wait

about a minute for the system to be primed with isoflurane gas.

5) Unlatch and open the Clean Box lid, remove the nose cone plug, and place the plug in its

holder. Remove the mouse from the anesthesia induction chamber and slide the muzzle of the
mouse into the nose cone (see the Pearl Imager Operator's Manual).

Make sure the surface is dry

before placing a mouse on the imaging bed.

IMPORTANT:

Observe the tail of the mouse and make certain the tail will not be pinched

between the Clean Box lid and imaging bed when the lid is closed.

6) Close and latch the Clean Box lid, then install the Clean Box cover on the Clean Box to prevent

touching the window or spotting the window with liquids.

7) If using the Pearl Docking Station, undock the Clean Box and move it to the Pearl Imager ima-

ging drawer and lock the Clean Box into the imaging drawer.

IMPORTANT:

Move the Clean Box quickly.

Anesthesia gas does not flow while the Clean

Box is undocked.

8) Remove the Clean Box cover to expose the window, close the imaging drawer, and acquire an

image as described in the Pearl Imager Operator's Manual.

9) After acquiring images, open the imaging drawer, undock the Clean Box and move it back to

the mouse holding facility or recovery chamber where the mouse can be removed.

10) When the imaging session is complete, replace the Clean Box cover to protect the Clean Box

window during storage.

Maintenance of the Clean Box Window

Proper care and maintenance are important to preserve the optical characteristics of the Pearl Clean
Box window. The following instructions are recommended for the proper care of the window.

Scratches

The window top and bottom surfaces are treated with a hard coating that aids in scratch prevention
under normal use. However, the window can still be scratched by dirt or other foreign objects.
Reasonable care should be taken to avoid scratching the window surfaces. Do not use paper towels
or similar products to clean the surfaces. Compressed, clean dry air is the preferred method of
removing foreign debris. If compressed air does not remove the debris, a soft lint-free optical
cleaning tissue should be used.

Smudging

Due to the light transmission characteristics of the window, it will be very easy to see smudges on
the window surfaces. To help prevent smudging, the exterior surface of the window has an
anti-smudge coating. For optimal optical performance, the interior window surface does not have

Page 12

Summary of Contents for 5700-DS

Page 1: ...Pearl Trilogy Accessories Manual...

Page 2: ......

Page 3: ...This product model number 5700 DS is a CE marked product For conformity information contact LI COR Support at http www licor com biotechsupport Outside of the U S contact your local sales office or d...

Page 4: ...nt NOTE Notes contain additional information and useful tips IMPORTANT Information of importance to prevent procedural mistakes in the operation of the equipment or related software Failure to comply...

Page 5: ...d by copyright All rights are reserved No part of this document may be photocopied reproduced or translated to another language without prior written consent of LI COR Inc Printing History Publication...

Page 6: ...Box Description 9 Installation 9 Connecting a HEPA Filter to the Docking Station 9 Connecting a HEPA Filter to the Pearl Imager 10 Nose Cone Installation and Replacement 10 Sanitizing the Pearl Clean...

Page 7: ...on trol to a Pearl accessory imaging bed outside of the Pearl Imager allowing animals to be prepared for imaging at an external station l Warms the heater plate in any of the Pearl accessory imaging b...

Page 8: ...instructions dans ce chapitre pour vous assurer que le port d chappement est reli un filtre charbon que le syst me d anesth sie fourni par l utilisateur est correctement reli et que le cone de nez est...

Page 9: ...UST on the rotameter to the port labeled OUT on the back of the docking station 5 If using the Pearl Clean Box connect one of the provided HEPA filter assemblies packaged with the Clean Box to the por...

Page 10: ...rage de Pearl n est compatible qu avec les syst mes d anesth sie qui fournissent le gaz isoflurane Le d bit et la pression dans l instrument ne devraient pas exc der 1 litre par minute et 6 9 kPa resp...

Page 11: ...UST port Recommendations for monitoring and replacement of the charcoal filter are given in Chapter 3 of the Pearl Imager Operator s Manual Figure 1 6 The included charcoal filter or a user supplied c...

Page 12: ...structions for the specific imaging bed you are using WARNING Make sure a black nose cone plug is tightly inserted in the nose cone before turning on the anesthesia gas Failure to insert a nose cone p...

Page 13: ...the adjustment knob on the rotameter to set the flow rate to 0 5 liters per minute typical and start gas flow from isoflurane vaporizer or equivalent Allow 1 minute to prime the system with isoflurane...

Page 14: ...ire or loose connections Periodically inspect external tubing for cracking or damage that could cause leaks Docking Station Specifications Docking Station Operating Conditions For Indoor use only oper...

Page 15: ...the anesthesia gas When the Clean Box is undocked the gas ports automatically close The Pearl Clean Box window is a specially coated cell cast acrylic window for optimum light transmission and durabi...

Page 16: ...ion after it is plugged in 3 Connect the opposite end of the HEPA filter assembly to the provided anesthesia supply tube provided with the Pearl Imager Nose Cone Installation and Replacement Nose cone...

Page 17: ...the imaging bed surface with 0 5 chlorine bleach Ethanol or isopropanol can be also used as a cleaning solution for applications where chlorine bleach is not required If necessary clean any fingerprin...

Page 18: ...IMPORTANT Move the Clean Box quickly Anesthesia gas does not flow while the Clean Box is undocked 8 Remove the Clean Box cover to expose the window close the imaging drawer and acquire an image as des...

Page 19: ...rove insufficient use LI COR Optical Tissue and LI COR Optical Cleaner Apply a few drops to an optical tissue and gently clean the window with a circular motion Follow with a second dry tissue to remo...

Page 20: ...iosciences GmbH Siemensstra e 25A 61352 Bad Homburg Germany Phone 49 0 6172 17 17 771 Fax 49 0 6172 17 17 799 bio eu licor com LI COR Biosciences UK Ltd St John s Innovation Centre Cowley Road Cambrid...

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