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8. All ports that are not connected to a chamber, or

used to supply/vent air from the flasks should remain
capped with the black plastic caps supplied with the
LI-8150. If these have been misplaced, replacement
caps can be ordered from LI-COR using part #620-
08298 and 620-08299. One of each will be needed for
every unused port.

Powering the system

The 8150-770 AC Power Supply can be used to supply
power to the LI-8150 and LI-8100A, as well as the
auxiliary pump used for continuously flushing the flasks.
The 8150-770 is capable of providing up to 4.5 Amps at
12 VDC.  When the auxiliary pump is installed as
described in this application note the input voltage to the
LI-8150 should not exceed a nominal 12VDC.

Software Setup

There are some software configuration changes that must
be made to use the LI-8150 and LI-8100A for multi-
plexed flask measurements. The bulk of these changes
are made under the Chamber tab of the Multiplexer
Configuration window and relate to defining the appro-
priate volumes and areas for the flux calculations.

1. Under the 

Setup

 drop down menu in the main

LI-8100A Windows interface software window, select

Measurement Configuration

 (Figure 10).

2. Select 

Port Setup

 from the Multiplexer Configura-

tion window.

3. In the 

Chamber 

tab, select 

Custom 

from the 

Cham-

ber:

 drop-down menu and enter the chamber

volume in cubic centimeters. Be sure to include the
volume of the tubing used to connect the flask to the
LI-8150 in the chamber volume

1

.

4. Make sure the closed signal check box is unchecked

and that the chamber offset is set to zero.

5. Select the chamber air temperature source from the

Temp Source dropdown menu. If the temperature
source is measured by a different port, skip this and
recompute with the correct temperature later. If
measurements are made at near ambient tempera-
tures a measurement of chamber air temperature
may not be required.

Figure 9.

 Mason jar lid fitted with plumbing

connections and a soil temperature thermistor. Two 8
mm holes are drilled in the lid to install the quick
connect bulkheads and a 12 mm hole is drilled to
install the grommet for the  thermistor.

either side of the lid, evenly spaced between the
larger hole and the edge of the lid (Figure 9).

2.

Place a seal washer (part #167-07256) on the

 

threaded portion of a female quick-connect bulk-
head fitting (part #300-07126) and install it in one of

 

the 8 mm holes using the nut and washer provided

 

with the fitting.  Repeat this procedure for the other

 

mm hole using a male quick-connect bulkhead

 

fitting 

(part #300-07127) (Figure 11).

3.

Insert a rubber grommet (part #196-10534) into the

 

12 mm hole.

4.

Gently push a soil temperature thermistor (part
#8150-203) through the hole in the grommet and

 

then pull back slightly to seal it.  A small amount of

 

silicone grease (part #210-01958-1) can be used to

 

ease insertion of the thermistor. To get a good seal

 

the 

grommet should fit tightly in the hole and against

 

the 

thermistor.

5.

Two sections of Bev-a-line tubing are required to

 

connect each chamber to the LI-8150.  Cut the

 

sections to the desired length and fit one end of each

 

tube with a female quick-connect (part #300-0712

5

)

 

and the other end with a male quick-connect (part

 

#300-0712

4

).

6.

Measure and record the length of each tube.  This

 

information is very important and will be required

 

later when the chamber volume is entered for use in

 

the flux calculations.

7.

Attach the tubes to the fixtures on the lid of each

 

flask, and then to the selected port on the LI-8150.

1

To calculate the volume of Bev-a-line IV, multiply the length of

tubing used (in centimeters) by 0.0792.  This will give volume in
cm

3

.

Soil temperature
thermistor

Female quick-connect
bulkhead (300-07126)

Male quick-connect
bulkhead (300-07127)

Rubber grommet
(196-10534)

Seal washer

7

Summary of Contents for LI-8100A

Page 1: ...early in half because the increased volume serves to dilute the change in CO2 mole fraction within the system As dC dt decreases the potential for error in the measurement due to instrument noise incr...

Page 2: ...s the mechanism by which CO2 and water vapor are exported from the system between measurements preventing condensation and excessively high CO2 mole fractions from developing in the flask The flushing...

Page 3: ...o brass plugs from the right hand upper and lower solenoid manifolds 2 Using an adjustable wrench replace the three brass plugs with quick connect elbows part 300 07474 and the one steel plug with a q...

Page 4: ...for flask measurements bottom right The flow path used during measurements is shown with blue arrows and the flow path used to flush flasks between measurements is shown with red arrows In both diagra...

Page 5: ...ver plate from the instrument by loosening the two thumb screws Figure 7A Mount the pump in the LI 8150 case by attaching the mounting plate to one of the two threaded holes normally used to attach th...

Page 6: ...thod 2 If dedicating a port to vent chamber exhaust and supply air to the auxiliary pump is undesirable the supply exhaust lines can be routed out of the LI 8150 by leaving the case open If using this...

Page 7: ...ments are made at near ambient tempera tures a measurement of chamber air temperature may not be required Figure 9 Mason jar lid fitted with plumbing connections and a soil temperature thermistor Two...

Page 8: ...as measured with a thermistor connected to V2 A volume correction can be made for the sample volume by entering a negative value for Extension Tube Volume cm3 Soil CO2 Flux System and LI 8150 Multiple...

Page 9: ...on air trapped in the measurement loop from the previous measurement with the air in the flask Figure 12 The change in CO2 mole fraction with time calculated for various deadband lengths using the dat...

Page 10: ...lated soil samples Jones and Kielland 2002 Haney et al 2008 and monitoring carbon fluxes from attached leaves and fruit Araki et al 1998 Kitano et al 1997 References Araki T M Kitano M Hamakoga and H...

Page 11: ...8100A 8150 770 01 8150 661 8150 203 8150 670 16 or 8150 670 8 196 10534 210 01958 1 9981 173 9981 177 9881 204 140 04315 8150 250 300 07124 300 07125 300 07126 300 07127 167 07256 300 08118 300 07474...

Page 12: ...licor com envsupport UK licor com LI COR Biosciences Global Headquarters 4647 Superior Street Lincoln Nebraska 68504 Phone 1 402 467 3576 Toll free 800 447 3576 Fax 1 402 467 2819 envsales licor com...

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