Voltage limiting
For most electrophoresis methods resistance increases throughout the run. Limiting the
voltage provides the following advantages:
•
Current and power decrease throughout the run, providing an improving margin of
safety over time.
•
The same voltage setting can be used regardless of the number or thickness of gels
being electrophoresed.
Current
limiting
Discontinuous buffer systems and, to a lesser extent, continuous systems increase
resistance during the run. If you use the current limiting setting on the PowerEase
®
90W
Power Supply, the voltage will increase as resistance increases to satisfy Ohm’s law
(V=IR). If no voltage limit is set and a local fault condition occurs, such as a poor
connection, very high local resistance may cause the voltage to increase to the maximum
capacity of the power supply. This may lead to local overheating and damage to the
electrophoresis cell or create unsafe conditions. When operating under constant current
conditions, set a voltage limit on the power supply at or slightly above the maximum
expected voltage.
PowerEase
®
90W Power Supply User Guide
9
Summary of Contents for PowerEase 90W
Page 19: ......