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5) The
LUNA™ will show you the progress bar as shown below. Do not turn off the LUNA.
<Calibration
– Step 1>
5.3.3 Step 2 Calibration
1) After Calibration Step 1, a new pop-up box will come on the screen as shown below.
<Calibration
– Step 2>
2) This step is for calibration with Trypan Blue dye. Prepare a calibration sample by mixing 10 µl
Trypan Blue Stain with 10 µl of Phosphate Buffered Saline (PBS). Load 10 µl of the mixed sample on
the slide, and insert it into the slide port.
3) Press
the “Start” button.