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5.5 Troubleshooting
Problem
Possible Cause
Solution
Inaccurate cell
count
Unfocused cell image
Ensure that the cell image is on an appropriate
focus by using the focus knob. You may use
the “Zoom-in” button when adjusting the cell
image. Make sure that live cells have bright
centers and dead cells have dark/blue centers.
Clumped cells
Make sure that cells are not clumped. The
more single cells, the better counting results.
Cell concentration range
The cell concentration range of the instrument
is 5x10
4
- 1x10
7
cells/mL. Make sure that the
sample is in this range. The sample may need
more cells or need to be diluted.
Counting slide insertion
Ensure that the counting slide is inserted
completely into the instrument. When the slide
is inserted, a soft click can be heard.
Sample loading
If the counting slide is over- or under-loaded
with the sample, it may affect counting results.
The optimal amount of sample is 10 - 12 µl of
cell suspension.
Malfunction of optical
components
Any of the optical components may be
damaged. Or, the objective lens may be dirty
due to dust, spilled samples, or unknown
causes. Please contact your local supplier.
Damaged counting surface
Make sure that the counting area of the slide is
transparent before loading the sample. Wear
gloves while handling the slide.
Data transfer and
saving
Incorrect USB drive
Use the USB drive supplied with the
instrument. Or, make sure that your USB drive
is compatible with the instrument. The version
of the USB drive must be 2.0. Some types of
USB drives are not detected or compatible with
the instrument.
Too many files in the USB
drive
When there are too many saved files on the
USB drive, reading and writing by the counter
may slow down.
