logos biosystems Luna II, Manual

Page 1: ...1 Catalog No L40001 L40002 Document No L44001 Revision Date December 02 2014 SW Version 1 4 4...

Page 2: ...2 IMPORTANT The LUNA II Automated Cell Counter is a Laboratory Electrical Instrument for Scientific Research Use Only BUT NOT A MEDICAL or THERAPEUTIC or IN VITRO DIAGNOTICS DEVICE...

Page 3: ...hanging Options for Cell Counting Counting Options 22 2 6 Setting the Date and Time 25 2 7 Calibrating LUNA II Calibrate 26 2 8 Updating the Software Update Software 30 Chapter 3 Protocol Setting 31 3...

Page 4: ...Results 55 Chapter 7 Maintenance and Troubleshooting 58 7 1 Turn On Off LUNA II 58 7 2 Cleaning 58 7 3 Calibrating the Touchscreen 59 7 4 Calibrating LUNA II 59 7 5 Updating the Software 59 7 6 Troubl...

Page 5: ...wer cord to the wall outlet as well as the instrument Always turn off the instrument before disconnecting the power cord and or moving the instrument 6 Ensure that the power cord is firmly plugged int...

Page 6: ...dinances of your region for proper disposal provisions The CE mark indicates that this instrument conforms to all applicable European Community provisions for which this marking is required Users must...

Page 7: ...l surface Make sure that no damage or contamination occurs on the optical surfaces of the slide 4 After mixing the cell sample with trypan blue stain perform cell counting as soon as possible within 3...

Page 8: ...tions Operating Power 100 240 VAC 1 2 A Frequency 50 60 Hz Electrical Input 12 VDC 3 3 A Installation Site Indoor use only Operating Temperature 10 35 Maximum Relative Humidity 20 80 Altitude 2 000 m...

Page 9: ...una Cell Counting Slide Third insert the slide into the slide port of the instrument and chose Autofocused Counting or adjust the focus by clicking arrows to get an appropriate cell image Last press t...

Page 10: ...ntegrated thermal printer provides concise Cell Count Report facilitating record keeping Innovative counting slide LUNA II adopts an innovative counting slide made with T BOND technology without using...

Page 11: ...ypan Blue Stain 0 4 2 x 1 ml Luna USB drive 1 After receiving the product package please immediately unpack it and check the components listed above to ensure that all parts are included and no damage...

Page 12: ...5 MP Image Type TIF format Optimized for LUNA II only Software LUNA II software www logosbio com Documentation PDF report Processing Time Nominal time for cell counting is less than 10 without autofoc...

Page 13: ...used to turn on the instrument Counting slide port is used to insert the Luna Cell Counting Slide containing sample to analyze The front USB port provides easy to access data retrieving to transfer 1...

Page 14: ...l counting results 1 5 5 Luna Cell Counting Slide The Luna Cell Counting Slide is a plastic disposable cell counting slide consists of 2 chambers labeled as A or B that can be used for the same sample...

Page 15: ...r end of the power cord into an electrical outlet after checking the outlet configuration in your local area Turn on the instrument using the power button located on the front of the instrument Initia...

Page 16: ...16 LOADING sign of the LUNA II Automated Cell Counter The Start Up screen will be displayed on the touchscreen as shown below in 2 2 See Section 7 1 for Turn on and Turn off the instrument...

Page 17: ...rt Up screen will show 5 compartments Power icon count review protocol and settings The Power icon in the Start Up screen can be used to turn off the instrument See Section 7 1 for further information...

Page 18: ...arameters in the Settings menu described below Press settings located on the right of the Start Up screen as below Then the following Settings screen will be displayed In the Settings screen users can...

Page 19: ...ge options for cell counting by pressing Counting Options Set the date and or time by pressing Date Time Re calibrate the instrument by pressing Calibrate Update the software by pressing Update Softwa...

Page 20: ...hscreen This function can be used when the response of touchscreen is abnormal On the Settings screen press Calibrate Touchscreen as below Now users can see the following screen If users want to calib...

Page 21: ...mark again The mark will move to lower right corner of screen Press it again The cross mark will further move to lower left corner and center of screen after each touch After touch the cross mark in...

Page 22: ...uld be set to value 2 Without Trypan Blue When samples do not contain trypan blue stain turn on this option and follow the directions in the message boxes For cell counting with this option please mak...

Page 23: ...ing Options screen current option will be marked as blue mark as below The counting option can be easily changed by pressing un selected option If Without Trypan Blue is chosen the following screen wi...

Page 24: ...lue is chosen the following sign will be displayed The Counting Option is simply changed by pressing OK Otherwise press Cancel to return the previous option Press in the upper left corner to move to t...

Page 25: ...the local time of Korean Once the date and time are set no additional setting is required for routine laboratory use On the Settings screen press Date Time as below The following screen will be displ...

Page 26: ...Counter provides easy to use automatic self calibration of background On the Settings screen press Calibrate as below The following screen will be displayed For the Calibration Step 1 the counting sl...

Page 27: ...led water phosphate buffered saline PBS or plain medium Put 10 l of diluted trypan blue solution into the chamber of new cell counting slide see Section 4 3 After completion of the Calibration Step 1...

Page 28: ...n Step 2 Following screen will be displayed with increasing red bar over the time Do not turn off the instrument After completing calibration the instrument will display following sign on the screen P...

Page 29: ...29 Now the date of last calibration is changed Compare to the Settings screen in page 26...

Page 30: ...is software should be saved into the root directory of the USB drive Before starting download the new software into the Luna USB drive and connect the USB drive to the USB port of the instrument Press...

Page 31: ...on Factor is used to automatically calculate the concentration of cells in original sample from the cell counting result Adjusting Dilution Factor will be helpful for users handling high density cells...

Page 32: ...can be adjusted with 1 m of increase per step Declustering Level Cultured mammalian cells may form clumps during culture or handling Declustering function of LUNA II provides efficient detection of c...

Page 33: ...protocol as below In the Protocol screen users can identify all the parameters as below The selected protocol is marked with white letters and blue back ground in the left panel of screen The value of...

Page 34: ...34 Note The factory setting DEFAULT protocol cannot be modified Press other protocol or New Protocol and press Load as below Now LUNA II display following sign on the screen...

Page 35: ...ected protocol press Delete To edit the selected protocol press Edit After pressing Edit the arrows upward and downward will be activated and marked as solid arrows as below Press the arrows to edit t...

Page 36: ...s can see the Save as screen as below Using keyboard in the screen put the desired name and press Save in the lower right corner Now new protocol named James is displayed in the Protocol panel of Prot...

Page 37: ...3 Selection of Protocol To change protocol to be used press the protocol button in the Set Up screen Select desired protocol by pressing the protocol name and press Load to apply selected protocol to...

Page 38: ...following sign on the screen Now the instrument is ready to count cells with selected protocol IMPORTANT Selecting the protocol name does not mean changing protocol to be used To use selected protocol...

Page 39: ...creen users can identify the Staining option and selected protocol in the upper panel of screen To change the Staining option see Section 2 5 Changing Options for Cell Counting To change the Protocol...

Page 40: ...on with 10 l of Trypan Blue Stain in an appropriate tube by gentle pipetting up and down 4 3 Loading Samples into the Luna Cell Counting Slide Hold the edge of the slide and load 10 12 l of the mixed...

Page 41: ...e inserted chamber upon counting Note After inserting the slide LUNA II only reads the inserted chamber Note Make sure that the counting slide is not inserted upside down Now users can see the cells o...

Page 42: ...en and moving the finger or stylus pen on the image Two small red boxes in the upper left corner of cell image represent the entire field and field of view respectively The outer box represents the en...

Page 43: ...of the buttons will be inactivated and the bar which is indicating the progress of cell counting will be appeared on the screen as below In general the counting takes 15 to 22 seconds LUNA II will dis...

Page 44: ...II Automated Cell Counter provides 1X 2X and 4X magnification of the image The captured image is also able to be navigated by pressing the screen and moving the finger or stylus pen on the image Two...

Page 45: ...es as below Note This Tag function is one of the distinct tools of the LUNA II Automated Cell Counter This function allows the user to review the data immediately to determine the accuracy of the coun...

Page 46: ...w On this screen users can review the distribution of cells according to their sizes Green bars represent the live cells and red bars represent dead cells The histogram will change by pressing two but...

Page 47: ...tion of dead cells without live cells as below In addition when pressing Cell number on the left side of histogram the LUNA II Automated Cell Counter also display the cell concentration The LUNA II Au...

Page 48: ...f screen as below The lower and upper limit can be activated by pressing as above and adjusted by pressing the icons with arrow head The gating function will be helpful to analyze the cells from co cu...

Page 49: ...the results press Save Print in the Cell Counting Results screen as below The Save Print screen will be seen as below In the upper part of screen three items will be displayed in inactive status Items...

Page 50: ...ally users can put the date and time by simply clicking Add Date Time in the lower right corner of screen Now users can save the selected data by pressing Save in the lower right corner of screen IMPO...

Page 51: ...eport will be displayed as below Cell Count Report File name HL 60 05092014 1 3350605 Date 05 Sep 2014 13 35 Cell count results Total 8 16x10e5 cells mL Live 6 63x10e5 cells mL Dead 1 53x10e5 cells mL...

Page 52: ...to activate the Dilution Calculator as below The Dilution Calculator initially shows the measured concentration of total cells from cell counting Users can select the Current Concentration of Total L...

Page 53: ...tofocusing With autofocusing function the LUNA II Automated Cell Counter achieves true automation of cell counting Users count cells by simply insert the cell counting slide and pressing Count without...

Page 54: ...nt to focus the image manually with various purposes The LUNA II Automated Cell Counter also provides manual focusing function Users can adjust the focus manually by simply clicking the arrow heads up...

Page 55: ...previous results without additional devices To review the previous results the USB drive that contains the previous results should be connected to the USB port of the LUNA II Automated Cell Counter U...

Page 56: ...the file name the previous cell counting results will be displayed on the right side of screen as below The captured image with tags can be also reviewed by pressing the small image under the Results...

Page 57: ...nts function users can overview the list of previous counts with summarized results as below This list can be exported into USB drive as a CSV format The LUNA II Automated Cell Counter can store up to...

Page 58: ...and continuously it may need to be cleaned or decontaminated to remove any dirt or dust on the surface of the instrument Be sure to turn off the LUNA II Automated Cell Counter and disconnect the power...

Page 59: ...rument this may cause severe damage to the instrument or give an electric shock to users Note do not use an abrasive solution or a bleach solution that can cause scratches on the outer surface or the...

Page 60: ...er Damage or contamination of counting slide Make sure that the counting area of the slide is transparent before loading the sample Wear gloves while handling the slide Data transfer and saving Incomp...

Page 61: ...one software version Delete software with previous versions from the USB drive before downloading new software Incorrectly saved or damaged software First make sure that the USB drive works well and...

Page 62: ...omated Cell Counter without printer each L12001 Luna Cell Counting Slides 50 slides 100 counts 1 box L12002 Luna Cell Counting Slides 500 slides 1 000 counts 10 boxes L12003 Luna Cell Counting Slides...

Page 63: ...product and its components by a party for monetary or other consideration including but not limited to a product manufacture b providing a service information or data c therapeutic diagnostic or prop...

Page 64: ...labs This limited warranty does not cover refund replacement and repair incurred by accident abuse misuse neglect unauthorized repair or modification of the Instrument This limited warranty will be in...

Page 65: ...urpose with regards to this Instrument To obtain service during the Warranty Period contact your local supplier or the Company s Technical Support team OUT OF WARRANTY SERVICE Please contact your loca...

Page 66: ...or email Our regional suppliers are listed on our web page www logosbio com Logos Biosystems Inc Address Suite 930 Doosan Venturedigm 126 1 Pyungchon Dong Dongan Gu Anyang City Gyunggi Do Republic of...