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2.5 Changing Options for Cell Counting (Counting Options)
The LUNA-II
™ Automated Cell Counter provides two options for cell counting: “With Trypan Blue” or
“Without Trypan Blue”. This function provides inter-change of these options.
Option
Description
With Trypan Blue*
This option can be used for regular bright field counting, when cell
samples are mixed with 0.4% trypan blue stain in a 1:1 ratio. This option
can generate cell viability data.
For cell counting with this option, the
dilution factor in the Protocol should be set to value
“2”.**
Without Trypan Blue
When samples do not contain trypan blue stain, turn on this option and
follow the directions in the message boxes.
For cell counting with this
option, please make sure that the dilution factor in the Protocol
should be set to value
“1”.**
*The cell counting parameters of LUNA-II
™ are optimized with the use of trypan blue. Low contrast from no
use of trypan blue stain may cause abnormal results.
**The
“Dilution Factor” in the “Protocol” is not changed automatically. After changing the “Counting Options”,
the
“Dilution Factor” should be changed manually. Improper use of the Dilution Factor causes incorrect
calculation of cell concentrations.
On the Settings screen, press [Counting Options].
