Special methods
33
NANOCOLOR
®
UV
/
VIS
II and
NANOCOLOR
®
VIS
II V 1.1 / 10.2017
In the "Enter values" mode, one can create a calibration curve by manually entering the
known concentration values and corresponding absorbance value pairs of standard
solutions. The "Measure standards" mode enables one to create a calibration table by
entering the concentration of the standard solutions and then measuring the corresponding
absorbance values. In both cases, the concentrations of stock solutions and the absorbance
values are plotted in a diagram. The calibration curve is charted, and all relevant statistical
data and the calibration function are calculated and displayed. A calibration function to the
4th degree can be plotted. Before starting the measurement, all data for the method must
have been filled in (see Figure 35).
Figure 35: Entering samples for the measurement of a calibration function
Press the
icon to enter the particular concentration on the numeric keypad.
Unwanted concentrations can be deleted from the list by pressing
. To create a
calibration, at least three concentration-absorbance value pairs must be entered. To increase
the statistical certainty level of the calibration function, a multiple determination can also be
performed for the respective concentrations. To do so, enter the desired value of the
concentration. The absorbance and concentration values will be entered in the correct
columns when the selection button above the table is pressed.
Wavelength:
Enter the wavelength of the measurement using the numeric keypad and
confirm your entry with OK. A wavelength in the 190 nm - 1100 nm range
(
NANOCOLOR
®
UV
/
VIS
II) or in the 320 nm-1100 nm range (
NANOCOLOR
®
VIS
II) can be
entered.
Unit:
Enter a unit via the text keypad, which will be shown when the result is displayed.
Confirm the entry with Enter. No more than 10 characters are allowed.
Precision:
Enter the desired number of decimals using the keypad and confirm your entry
with OK. The measurement result will be displayed with the selected number of decimals.
Cuvette size:
Select the cuvette size from the list box that pops up. The photometer detects
automatically whether the correct cuvette size is being used.
Reference:
Select the desired reference for the measurement. In addition to water, the
reagent blank value can also be chosen here. If values are measured, the device will ask for
the insertion of the corresponding reference cuvette
.
The specification appears in the report
on the special method.
Summary of Contents for NANOCOLOR UV/VIS II
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