Malvern Nano Series, Basic Manual

Page 1: ...and MRK1378 01 Malvern Instruments Limited Enigma Business Park Grovewood Road Malvern Worcs WR14 1XZ U K Tel 44 0 1684 892456 Fax 44 0 1684 892789 www malvern com Q SelBBBbbBasic Guide Zetasizer Malv...

Page 2: ......

Page 3: ...Zetasizer Nano Basic Guide MAN0486 Issue 1 0 September 2012 English Zetaszier Nano Basic Guide Man0486 1 0 book Page i Monday September 10 2012 2 19 PM...

Page 4: ...without the express written permission of Malvern Instruments Ltd Head office Malvern Instruments Ltd Enigma Business Park Grovewood Road Malvern Worcestershire WR14 1XZ United Kingdom Tel 44 0 1684 8...

Page 5: ...5 Computer specification 2 5 Health and safety Introduction 3 1 General safety issues 3 1 Power cords and Power safety 3 5 Moving the system 3 7 Maintenance of the instrument 3 8 Sample handling warn...

Page 6: ...Page ii MAN 0486 Performing a quick measurement 4 14 Next 4 25 Appendices Specifications Optical unit A 1 Chemical compatibility A 4 Regulatory Statements A 5 Zetaszier Nano Basic Guide Man0486 1 0 b...

Page 7: ...ture instruments have HT on the main instrument label High Temperature and other build options exist for all the above instruments Zetasizer Nano instrument Model number Measurement types Nano S Red b...

Page 8: ...2 Titrator and Degasser refer to the Titrator and Degasser user manual where necessary Access to the instrument Malvern personnel Malvern personnel service engineers representatives etc have full acce...

Page 9: ...es or sample dispersion units are referred to as MPT 2 Titrator the vacuum degasser or as the accessory or the dispersion unit The combination of the optical unit one or more accessories or dispersion...

Page 10: ...ction operation and maintenance of the dispersion units Help System Each key software window has a Help button giving information specific to it Additional Info Tips are also available whenever is sho...

Page 11: ...Contact the International Malvern Helpdesk if the local Malvern Instruments representative is not available Telephone 44 0 1684 891800 or email helpdesk malvern com Note This help line is primarily E...

Page 12: ...Chapter 1 Introduction to this manual Page 1 6 MAN 0486 Zetaszier Nano Basic Guide Man0486 1 0 book Page 6 Monday September 10 2012 2 19 PM...

Page 13: ...ion the system Environmental conditions The site must be Away from strong light sources e g windows Away from strong heat sources e g radiators Well ventilated for noxious samples On a horizontal vibr...

Page 14: ...gram shows the minimum recommended space required for a typical system using an MPT 2 Titrator and its computer IP rating Designed to meet IP41B Operational conditions 5 C to 40 C 41 F to 104 F Storag...

Page 15: ...er supply must be clean and filtered If necessary fit an Un interruptible Power Supply UPS to remove any spikes or noise The power requirements are Component Zetasizer Nano MPT 2 Titrator Vacuum degas...

Page 16: ...curring on the cell this occurs when the measurement temperature is less than the dew point of the ambient air surrounding the cell being measured This is particularly likely in humid climates The pur...

Page 17: ...n that may change the pH characteristics of the sample i e cause a pH drift If a Nitrogen supply is required it must conform to these specifications The Nitrogen supply must be dry free from oil and f...

Page 18: ...Chapter 2 Site requirements Page 2 6 MAN 0486 Zetaszier Nano Basic Guide Man0486 1 0 book Page 6 Monday September 10 2012 2 19 PM...

Page 19: ...enance of the instrument Sample handling warnings Disposal of the instrument General safety issues Warning Use of the system in a manner not specified by Malvern Instruments may impair the protection...

Page 20: ...rear panel Restricting airflow can damage the instrument or cause overheating Purge warnings MPT 2 Titrator Warning If a Nitrogen supply is used the system must be located in a well ventilated enviro...

Page 21: ...t in hazardous radiation exposure This diagram shows the location of the laser warning labels ill 8805 Zetasizer Nano Z S ZS S90 ZS90 specification Red laser Item Specification Light source He Ne Lase...

Page 22: ...a spillage occurs disconnect the power and scrupulously clean up before re applying power to the system Users who suspect powder or liquid has entered the covers should call a Malvern representative t...

Page 23: ...cord set requirements contact a Malvern representative General requirements These requirements apply to all countries The power cord must be approved by an acceptable accredited agency respon sible f...

Page 24: ...ion lead does not exceed the ampere rating of the extension lead Use caution when plugging a power cord into a multiple socket extension lead Some extension leads may allow a plug to be inserted incor...

Page 25: ...n the metal base plate Never lift an instrument by its covers If moving the instrument large distances we recommend repacking the instru ment in its original packaging Moving sample dispersion units I...

Page 26: ...nal serviceable parts Never attempt to remove the covers of the optical bench or an accessory or dispersion unit Removal of the covers invalidates the warranty and may expose the user to dangerous las...

Page 27: ...ween different types of sample Cross contamination between samples can seriously affect the results Please refer to the Zetasizer Nano Accessories guide for information on clean ing and maintaining th...

Page 28: ...glass Always test a new sample or dispersant for chemical compatibility before use After measuring hazardous samples scrupulously clean the system to remove any contaminants before making another mea...

Page 29: ...when the last user wishes to discard this product it must not be treated as general waste Instead it shall be handed over to the appropriate facility for the recovery and recycling of electrical and e...

Page 30: ...Chapter 3 Health and safety Page 3 12 MAN 0486 Zetaszier Nano Basic Guide Man0486 1 0 book Page 12 Monday September 10 2012 2 19 PM...

Page 31: ...eta measurement using the Zeta potential transfer standard that is included with the system A full description of the Zetasizer nano system the hardware and software can be found in the following manu...

Page 32: ...o key stages which are paused after the completion of each stage Manual measurements tend to be used for one off measurements or as part of method development i e establishing the optimal settings for...

Page 33: ...MPT 2 Titrator shown alongside can be included to form part of the overall system ill 8499 The Zetasizer Nano instrument Optical unit Positioned on the cover are two labels one to identify the instrum...

Page 34: ...ptical unit Cell area Rear Panel Cuvette holder Status indicator Cells and Cuvettes Cell access button Power input socket Serial number and Model number label Fuse holder Mod record Power switch Acces...

Page 35: ...middle of the Status indicator pressing the button will open the cell area lid Cell area Warning The system is capable of heating the cell to high temperatures Care should be taken when removing the...

Page 36: ...older is for storing the cells before and after use the cuvette holder swings out from the instrument and up to 12 cuvettes can be stored Cell area lid Thermal cap Electrodes Drain port Access channel...

Page 37: ...pplication Disposable polystyrene cuvettes Standard and Small volume Size and Zeta potential with dip cell Folded capillary cell Size and Zeta potential High concentration cell Size and Zeta potential...

Page 38: ...ular weight result It displays the results and allows reports to be printed The Zetasizer software application A typical screen is shown below The features and their function are described in the foll...

Page 39: ...cut copied pasted and deleted into their own or other measurement files View Menu The View menu selects which reports will be shown in the measurement file window and which Toolbars are to be dis pla...

Page 40: ...ays only parameters associated with zeta potential meas urements Record and Report tabs Measurement records are viewed with the Records view tab this gives a listing of all the measurement records in...

Page 41: ...icons The status bar gives an indication of the instrument s current operating state and an extended description of the tool icons If required use the View Toolbars Customise window to disable it On...

Page 42: ...the system ill 7905 Connect the USB cable to the connector marked USB on the rear panel of the Zetasizer Nano instrument Connect the other end of the cable to the USB connection on the computer Connec...

Page 43: ...ument is changed the following actions will be required Consult the software update notification supplied with the software Installing the software 1 Insert the Zetasizer software CD into the CD drive...

Page 44: ...ms correctly Unpack the Zeta standard Take the Zeta potential transfer standard syringe from the pack this document was in Run the Installation test macro Enclosed with the System is an installation t...

Page 45: ...it will test only zeta potential measurement skip the Size test procedure below Important Always hold the cuvette or cell near its top not the optical area near its base Fingerprints grease or dust on...

Page 46: ...between 10 and 15 mm This can be checked by placing the cuvette against the diagram on the inside of the cell area lid ill 7936 e Push the lid securely onto the cuvette ill 7937 Max 15mm Min 10mm Zet...

Page 47: ...ionised water to clean Flushing a few 10ml syringe full through the cell should suffice c Invert the cell ill 7938 d Slowly inject the Zeta potential transfer standard from its syringe into the cell...

Page 48: ...ll are completely immersed in the sample i Remove the syringe and insert a cell stopper in each port ill 7940 j Remove any liquid spilt on the outer part of the electrodes and the outside of the cell...

Page 49: ...l in the instrument following the procedure below then click OK Size test a Press the button to open the measurement chamber lid ill 7942 b Most cuvettes have a triangle or spot mark This must face to...

Page 50: ...irmly located then push down the chamber lid ill7944 Zeta potential test a Press the button to open the measurement chamber ill7942 b The cell has a weld line just behind the front surface as shown he...

Page 51: ...en inserted d Push the cell into the cell holder until it stops ill 7946 e Close the measurement chamber lid to cover the cell The test runs pass or fail Each test should take no longer than about six...

Page 52: ...ass If testing both size and zeta potential after the Size test the instrument prompts for the capillary cell for the Zeta potential test The instrument is only set up correctly if both tests pass Not...

Page 53: ...g ethanol or methanol as described ear lier then check that there are no scratches on it Wipe it dry with a lint free cloth we recommend camera lens cleaning pads Do not try to clean the optical area...

Page 54: ...verview Page 4 24 MAN 0486 Intensity PSD M report for a size test ill 8488 Zeta potential M report for a zeta potential test ill 8189 Zetaszier Nano Basic Guide Man0486 1 0 book Page 24 Monday Septemb...

Page 55: ...in a selection window Select the SOP to use and click the Open button 3 Follow any on screen instructions to run the SOP Clean and load the cuvette or cell exactly as described above for the self ins...

Page 56: ...Chapter 4 System Overview Page 4 26 MAN 0486 Zetaszier Nano Basic Guide Man0486 1 0 book Page 26 Monday September 10 2012 2 19 PM...

Page 57: ...as sample dispersant Nano S90 and ZS90 Range maximum diameter Minimum sample volume Minimum concentration protein Maximum concentration Measurement angles water as sample dispersant 0 3nm 10 microns 1...

Page 58: ...ZSP Standard Red CDRH and CE compliant Class llla laser product CDRH Class 3R laser product IEC60825 1 1993 A1 1997 A2 2001 Type HeNe gas laser Max output power 4mW Beam diameter 0 63mm 1 e Beam dive...

Page 59: ...cuvette holder closed Weight 19kg Power requirements AC 100 240V 50 60Hz Power consumption Max 100W Ambient operating conditions Humidity 10 to 35 C 50 to 95 F 10 to 90 non condensing Recommended comp...

Page 60: ...ealth and safety chapter Cell area As the sample measured is held within the cuvette or cell used all components within the cell area will only come into contact with the sample if spillage occurs The...

Page 61: ...reated as general waste Instead it shall be handed over to the appropriate facility for the recovery and recycling of electrical and electronic equipment By not discarding this product along with othe...

Page 62: ...ce received including interference that may cause undesired operation Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FC...

Page 63: ...s equipment This Class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A est conforme la norme NMB 003 du Canada VCCI acceptance Japan only The Voluntary Contro...

Page 64: ...Appendix A Appendices Page A 8 MAN 0486 Zetaszier Nano Basic Guide Man0486 1 0 book Page 8 Monday September 10 2012 2 19 PM...

Page 65: ......

Page 66: ...alvern Instruments Limited Enigma Business Park Grovewood Road Malvern Worcs WR14 1XZ U K Tel 44 0 1684 892456 Fax 44 0 1684 892789 www malvern com Quick start Self installation guide Zetasizer Malver...