Advanced protein features
Chapter 6
Zetasizer Nano accessories guide
Page 6-3
Once all of the groups have been recorded the
post
-mobility
size
measurement is performed in order to determine the state of the sample after
the electrophoresis measurement.
This completes the measurement process.
Diffusion barrier measurement technique
Protein mobility measurements should be used in combination with the
diffusion
barrier
measurement technique to further protect the sample.
It has been found that most of the damage that happens to protein samples, during
mobility measurements, happens at the electrodes. The diffusion barrier technique
protects the protein from damage by introducing a physical distance between the
sample and the electrodes. This is done by holding a plug of measurement sample
within a larger volume of the same buffer that contains no protein. This separates
the protein from the electrodes and prevents any protein-electrode interaction and
sample damage.
The sample sits at the bottom of the cell if the cell is handled carefully.
Advantages of this technique are that:
Many more measurements can therefore be made before aggregates start to
appear.
Only small volumes (20-50
μ
l) are required.
. Standard measure-
ment volume
Measurement sample
covers electrodes
. Diffusion barrier
measurement volume
Sample plug shown with
buffer between plug and
electrodes
ill ill8802
A
B
Summary of Contents for Zetasizer Nano Series
Page 2: ......
Page 3: ...Zetasizer Nano accessories guide MAN0487 Issue 1 1 April 2013...
Page 12: ...Chapter 1 Introduction and accessory range Page 1 6 MAN 0487...
Page 42: ...Chapter 2 General cells and cuvettes Page 2 30 MAN 0487...
Page 98: ...Chapter 5 Microrheology Page 5 20 MAN 0487...
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