Aligning the phase rings
To center:
Rotate 10X infinity plan phase contrast objective into the field of view, then set the Zernike phase
disc to position “10” to match the objective.
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through
the centering telescope, the dark and bright ring images should coincide with each other as shown
in the figures below. If the ring images can’t be observed clearly, focus the centering telescope first
and if this does not solve the issue then try to adjust the condenser by turning it up and down.
If the bright ring and dark ring images are not coincided as shown below, adjust the position of the
ring by moving the ring at the bottom side of the condenser with your fingers. Move it until bright
and dark ring images superimposed. Repeat for all objectives/ Zernike disk positions.
Not centered
Centered properly
Phase condenser
Phase objective
Centering telescope
Filters