Merck Millipore ReadyStream, User Manual

Page 1: ...www sigmaaldrich com User Guide ReadyStream System The life science business of Merck operates as MilliporeSigma in the U S and Canada...

Page 2: ...ular purpose with respect to any technical assistance or information that we provide Any suggestions regarding use selection application or suitability of our products shall not be construed as an exp...

Page 3: ...ed stock 19 Calibration of the peristaltic pump 21 Water pump calibration status check 22 Routine usage 23 Definition of the dispensing parameters 23 Selection of the dispensing parameters 23 Using a...

Page 4: ...ructions for further reading System overview The ReadyStream system is designed to facilitate work in microbiological culture media preparation and dispensing The ReadyStream dispensing unit is low in...

Page 5: ...rical shock and short circuit Before cleaning shut down the ReadyStream instrument and disconnect from the power source The power supply must be protected by a fuse below the main connection The elect...

Page 6: ...l Point of connection for the unit s water supply Barcode reader label Connection port for the unit s barcode scanner Power supply label Connection port for the unit s power supply The system power co...

Page 7: ...with FCC IC and EC as a class B equipment Connection 24 V jack output for accessory RS232 modified output to connect scanner RJ 45 output to connect media unit and dispensing unit Power source RDYSTRM...

Page 8: ...n home position F2 Peristaltic pump head F1 Outlet connector for the filter set B5 Venting B4 Marking plate B3 Power cord connector B2 RJ45 connector to media dispensing unit B1 RS232 connector to bar...

Page 9: ...ides R1 Extension tray screw R2 Main power switch R3 LED transillumination switch R4 Accessory port R5 Front cover pin R6 Guide for dispensing tubing L2 Front cover translucent L1 Deionized water inle...

Page 10: ...r magnet inside 5 Control panel 6 Dispensing holder 7 Height adjustment screw 8 Tube guide 9 Stand screw 10 Connector to media unit ON OFF Filter priming Bag filling Preset selection and storage Prese...

Page 11: ...re 5 Description of the ReadyStream filter set A B C C D E F G H I Description of the ReadyStream media bag A Filter connector B Dispenser connector C Silicone tubing D Silicone tubing for peristaltic...

Page 12: ...zation kit Installing the instrument Install the media unit on a bench or on a trolley supporting a mass of 30 kg Install the dispensing unit close to the media unit less than 1 m distance It is also...

Page 13: ...the pressure flow rate and temperature of the deionized water loop are within the range of the operational conditions of the ReadyStream instrument Notably if several instruments are connected to the...

Page 14: ...unit Dispensing unit Barcode reader Bag outlet set between guiding pins Filter set connection to preparation module Silicone tubing set in pump head 4 way valve set in rotary actuator Filter set conn...

Page 15: ...he number of remaining connections to media bags e g C6 connections the remaining volume of water e g V224 Liters and remaining lifetime e g 15 days are displayed Always use the same filter set on the...

Page 16: ...the dispensing unit The orange cap is to be inserted perpendicularly to the gray connector at the bottom side of the dispenser arm Do not run the instrument with the orange cap connected to the dispe...

Page 17: ...ed to scan and connect a media bag Priming of the filter set For optimal performance any air in the inlet tubing in the instrument and in the filter set must be removed Ensure the media unit is connec...

Page 18: ...necting a filter attached to a media bag previously fitted to the instrument perform 5 priming cycles to flush the filter and tubing with about 1 liter After priming the Prime LED is solid green to in...

Page 19: ...trol panel The mL LED flashes green The calibration value can be adjusted using the or buttons Press the DECIMAL button to highlight the digit to modify When properly set modification is stored by pre...

Page 20: ...bag filling e g leakage press CANCEL to stop the water pump safely The Filter Bag and Prog LEDs will flash red Filling cannot be resumed and therefore the media bag must be discarded When the bag is f...

Page 21: ...on step once the pump head is closed To ensure precision in media dispensing the peristaltic pump must be calibrated each time a new bag is installed on the instrument or each time the pump head is ac...

Page 22: ...ng Press the WATER button to start the flushing step When the Water LED is blinking green the user is prompted to stop the water pump Make sure to have a container on the dispensing unit to collect th...

Page 23: ...n page 25 0 5 L min 2 L min X Final concentration of the culture media dispensed by the instrument By default single strength 1 x media is dispensed and X 1 is not displayed 0 1 x 2 x If flow rate mod...

Page 24: ...urn green and the respective parameters are displayed The system is ready to dispense media or water Scanning a test data matrix The parameters can also be set by scanning a specific data matrix see d...

Page 25: ...gram the Preset or Scan LED is turned OFF Storing a customized program Any customized program or scanned data matrix can be stored as a new preset The maximum number of presets that can be stored is 4...

Page 26: ...ia LED turns back to solid green and the program parameters are displayed The system is ready for further dispensing Make sure to homogenize the test bag or container contents before incubation or fur...

Page 27: ...media bag and the filter with the system follow the process steps previously described to setup the consumables repeating at least five times the priming step to flush the tubing with approximatively...

Page 28: ...ranteeing the final temperature During the pre heating step the Water LED will blink orange and the display will indicate the current temperature of the water in the system and tubing and the remainin...

Page 29: ...ispensations of 3375 mL each within 3 days After 5 seconds the bag information is cleared and the current dispensing parameters are displayed again When scanning the label of a media bag other than th...

Page 30: ...l fluidic path If the system has not been used regularly for more than 2 weeks it is recommended to sanitize the instrument using a specific procedure Volume mL Temperature C Concentration x Flow rate...

Page 31: ...sanitization kit To start the sanitization process turn ON the instrument and scan a specific sanitization data matrix instead of the filter label then follow the instructions on the display Example...

Page 32: ...nsing water The screen then displays the number of rinsing cycles and the waiting time between each The Water LED will be flashing orange Press WATER to start the rinsing step The Water LED flashes gr...

Page 33: ...et water source pressure out of range Ensure that the water source can deliver at least 2 liters min of deionized water Check the strainer filter and clean if applicable Check that the input water pre...

Page 34: ...rature to be set and realized impact of inlet water temperature media bag temperature target temperature media bag concentration target concentration If temperature accuracy is not critical it is poss...

Page 35: ...ssistance Please check the product webpage whether an update of this user guide is available For more information visit www sigmaaldrich com techservice 2021 Merck KGaA Darmstadt Germany and or its af...