User Guide Version 2.60 I-Button
Low Volume
Samples
Please note:
Prior to each running a
test, the system will
perform autocalibration
(do not insert a
capillary until
instructed to do so on
the screen.)
If the sample is < 0.5 ml two options are available: Run as a low volume sample
and obtain just motility parameters or dilute the sample 1:2 (1+1) with
QwikCheck Dilution media and obtain a report of all parameters.
To run a low volume sample: Aspirate only 20 µl of sample into the motility
section of the capillary following the instructions in the Appendix section of this
User Guide: “Filling the SQA-V Capillary with a Low Volume Samples”.
Diluted
Samples
Please note:
See the appendix
section of this guide for
information about
dilution media.
Follow the instructions in the appendix section of this User Guide: Filling the
SQA-V Capillary with a Normal Volume Sample.
The testing cycle and test results will be the same as a normal volume specimen
(see screens below).
Revision 21_Sep_2015
15
Or the low volume sample can be diluted 1:2(1+1) with QwikCheck-Dilution
media:
FILL CAPILLARY – 20 MICROLITERS
CLEAN AND WIPE CAPILLARY
LOW VOLUME SPECIMEN
PLEASE SELECT SAMPLE TESTING OPTION:
DILUTE SEMEN 1:2 (1+1) WITH MEDIA
LOW VOLUME – 20 MICROLITERS ONLY
MOTILITY PARAMETERS ONLY
LOW VOLUME SAMPLE
INSERT CAPILLARY INTO CHAMBER
TEST RESULTS
MOTILITY PARAMETERS ONLY
MSC 18.5 M/ml
PMSC 8.3 M/ml
VELOCITY 5 mic/sec
SMI
26
TOTALS PER VOLUME
MOT SPERM 18.5M PROG SPERM 8.3M
LOW VOLUME SPECIMEN
PLEASE SELECT SAMPLE TESTING OPTION:
DILUTE SEMEN 1:2 (1+1) WITH MEDIA
LOW VOLUME – 20 MICROLITERS ONLY
MOTILITY PARAMETERS ONLY
LOW VOLUME SPECIMEN
1.
DILUTE SEMEN 1:2 (1+1) WITH MEDIA
2.
MIX SAMPLE THOROUGHLY
3.
FILL, CLEAN AND WIPE CAPILLARY
INSERT CAPILLARY INTO CHAMBER
