Revision 21_Sep_2015
31
User Guide Version 2.60 I-button WHO 5th
APPENDIX 1:
Filling the SQA-V Capillary with a Normal Volume Sample
Sample size, collection container and preparation:
1.
Sample volume should be
at least .5 ml
If sample volume is less
than .5 ml see Appendix 2.
2.
Sample container should be
wide-necked and deep enough
to
facilitate inserting the capillary into the sample at the bottom of the
container.
3.
The semen sample must be
completely liquefied and well mixed
prior to aspiration
. Gently rotate container to fully mix liquefied
specimen.
WARNING:
Do not shake nor use a pipette to aspirate and dispense
specimen in order to mix, otherwise air bubbles will form.
Figure 1
4.
Carefully check that liquefied, fully mixed specimen is free of air bubbles
(or that there is
an adequate amount of sample below the air bubbles) before immersing the capillary into the
specimen, thus ensuring that no air bubbles will be aspirated into the capillary.
Filling the capillary:
1.
Push the syringe piston in fully
. Place only thin part of the capillary
into the bottom of the sample while angling the sample container at
about 45 degrees (Figure 1).
2.
Placing two fingers below the piston head
pull the piston back slowly
while keeping the tip of the capillary well below the sample level
and below any surface bubbles
(Figure 1). Continue to aspirate the
sample until it appears in the Luer adaptor.
Figure 2
NOTE: Transferring the sample to a standard "tissue culture dish" (3 cm in diameter/1 cm deep)
will allow better visual control when filling the capillary as an intermediate step (see Figure 2).
