Revision 21_Sep_2015
43
User Guide Version 2.60 I-button WHO 5th
Appendix 12: Post-vasectomy Protocol
The SQA-V runs a five minute POSTVASECTOMY test that can detect the presence of a very small number
of motile cells. Once the automated test has been performed the user is given the option to follow the
POSTVASECTOMY protocol outlined below (also refer to the Appendix section of this guide) and "scan" the
testing capillary in the SQA-V visualization system.
By scanning through the depth of the testing capillary the user is able to identify and readily count immotile
cells and visually confirm automated test results. Clinical studies positively demonstrated that by
incorporating both the SQA-V automated AND the visualization system in the testing protocol, a very high
level of accuracy is obtained for identifying motile and non-motile cells in POSTVASECTOMY samples.
In order to obtain similar levels of accuracy it is imperative that the user strictly follow the manufacturer’s
protocol outlined below. Additionally, once the testing cycle is complete, the user has an opportunity to
document test results by capturing and archiving a video clip of the postvasectomy specimen using
V-Sperm™ software.
This test is highly sensitive to any movement. The SQA-V and the testing capillary should not be disturbed
in any way during the 5 minute testing cycle.
Fill BOTH sections of the SQA-V testing capillary (for stability during the 5-minute test).
If the specimen volume is not adequate to fill both sections, dilute it with Earle's Buffer and
multiply results by the dilution factor.
Follow the user guide for instructions on running a POSTVASECTOMY sample.
Run the automated 5-minute test for motility parameters.
Remove the capillary and insert it into the visualization system and "scan" ten fields of the SQA-V
capillary following the user guide instructions.
Enter the number of motile and immotile sperm cells visualized.
The final test results will report the greater number of cells found in the automated or visualization
test.
Leave the testing capillary in the visualization system.
Save the test to the SQA-V archive and import it to the V-Sperm GOLD software.
Following the V-Sperm user guide instructions, import the test into the V-Sperm data base and
attach a live VIDEO clip to the patient’s test record for documentation purposes.
NOTE:
If the SQA-V is reporting > 30 motile spermatozoa, a screen will indicate that a NORMAL
TEST should be run instead of POSTVASECTOMY > 30 motile spermatozoa is equivalent to
MSC > 2M/ml.
