micronit microfludics FLUIDIC CONNECT 4515, User Manual

Page 1: ...FLUIDIC CONNECT 4515 User Manual Version 1 5 Visiting address Phone Fax E mail Internet Colosseum 15 7521 PV Enschede The Netherlands 31 53 850 6 850 31 53 850 6 851 info micronit com www micronit com ...

Page 2: ...Fluidic Connect 4515 User Manual V1 5 2 ...

Page 3: ... 3 Step 3 Placing the Alignment Nuts 2 4 Step 4 Connection of Fittings 3 FRAME 4 QUESTIONS AND ANSWERS 5 TIPS AND TRICKS FOR CONNECTION 5 1 Tightening force 5 2 Stainless Steel Teflon Connection Kit 6 SPECIFICATIONS 7 PARTS LIST Fluidic Connect 4515 User Manual V1 5 4 5 5 5 6 7 12 13 15 15 16 17 18 3 ...

Page 4: ...in top and bottom parts These openings allow the chip to be inspected while in the chip holder using an inverted or standard microscope Multiple chips can be connected in parallel or in series for additional flexibility Keyfeaturesof FluidicConnect4515 User friendly leak free microfluidic connections Easy chip replacement thanks to Fluidic Slide Improved alignment of the microfluidic chip Improved...

Page 5: ...ction Kit that is used Check the parts list on page 18 to see if all the parts are included Figure 2 1 All the parts of Fluidic Connect 4515 Clean the chip holder and place the chip in the chip holder The user friendly design allows the user to push slide the chip into the chip holder over a flat surface see Figure 2 2 Make sure that the arrow on the chip cartridge directs to the holder Figure 2 2...

Page 6: ...nt ports You only need to slightly press the nuts into the ports to align the chip properly Do not over tighten the alignment nuts this might damage the chip The alignment nuts will position the chip such that the holes in the chip are concentrically to the ports in the chip holder Figure 2 3 Screw the alignment nuts gently into the chip holder Fluidicports Alignmentports MicroreactorChips 2 4 8 3...

Page 7: ...etter grip on the connection just proceed with step 2 Step 2 After finger tightening tighten the nut with the extender tool another 1 4 round for Flat Bottom connections Teflon Stainless Steel another 1 3 round for Nanoport connections Fused Silica Step 4Ais specifically for the Fused Silica Connection Kit 1 Adjustingcapillarylength The Nanoport ferrules and nuts supplied with the Fused Silica Con...

Page 8: ...ould not protrude Figure 2 7 2 The ferrule can be used in two different ways with the conical side down pressed into the conical hole of the chip lowest dead volume with the conical side up flat area of the ferrule will be pressed onto the glass for high pressure connections It is advised to use latex gloves during handling in order to prevent contamination of the ferrules Also rinse the ferrules ...

Page 9: ...t the capillaries are aligned with the channels Figure 2 9 Figure 2 9 Capillary correctly positioned above the channel When sealing with the conical side inside the hole which is optional the intensity of the colour of the seal will change by tightening the fittings The reason for this is the increasing contact area between the sealing rubber and the sidewall of the hole Fluidic Connect 4515 User ...

Page 10: ...g The flattened end of the ring should face towards the nut with the narrow end of the ferrule towards the ring Figure 2 10 For the untrained eye it can be hard to determine which side is narrower so double check if you are not sure The metal ring should be pushed onto the ferrule in that way ensuring concentricity of ferrule and ring Figure 2 11 Figure 2 10 Fittings order Figure 2 11 Ensure conce...

Page 11: ... as shown in Figure 2 13 Figure 2 13 Proper assembly of compression ring and ferrule 3 Makingfluidic connections Tighten the fitting in the appropriate fluidic ports see Table 2 1 by using the extender tool as shown in Figure 2 14 No extreme force should be necessary Figure 2 14 Tightening the connection Fluidic Connect 4515 User Manual V1 5 11 ...

Page 12: ...the chip from the bottom side The chip holder can be placed upside down in the frame Figure 3 1 Connect the tubing to any auxiliary equipment The chip holder frame allows the tubing to be guided straight down or to the sides Figure 3 1 Chip holder upside down in the chip holder frame Fluidic Connect 4515 User Manual V1 5 12 ...

Page 13: ... found there way into the chip Blocking with Nanoport ferrules is possible when the Nanoport ferrules are used with the conical side pressed in the chip hole and the fittings are tightened too much Release the fittings slightly and check whether a flow is present Theconnectionleaksduringtesting When the ferrules are not assembled straight onto the glass chip there can be a leakage Assemble the con...

Page 14: ...ls solely used for manufacturing purposes When you see fluid in these degassing channels this does not mean that the chip is broken Normally there is no water in the degassing channels but it is possible that during cleaning or after previous leakage fluids flows in these degassing channels from the sides of the chip When fluids are visible check the fluidic connections to see if there is no leaka...

Page 15: ...ysthatIshouldtightentheconnectionfingertight howdoIknowif theconnectionisfingertight Note that over tightening can break the glass chip especially when working with a thin bottom chip like the R50 332 3 or the X3550CH 3 chip Use the following protocol to avoid breaking of microfluidic chips 1 To tighten finger tight you should twist the connection with the tip of your thumb and index finger until ...

Page 16: ...tcanbewrong 1 Make sure the connections are finger tight see 5 1 Tightening Force if so DO NOT tighten the connections any further it might break the chip 2 Check if there is no tension on the tubing causing the tubing to exert a force on the seal 3 If the tubing exceeds the ferrule edge this can cause the problem see figure 5 2 Fluidic Connect 4515 User Manual V1 5 A B 16 Ferulle and tube incorre...

Page 17: ... l x w x h 100 x 75 x 24 mm Material of frame POM Polyoxymethylene FusedSilicaConnectionKit Dimensions capillaries 360 375 µm OD 150 µm ID Material nuts PEEK Polyether ether ketone Material fittings Perlast Teflon ConnectionKit Dimensions capillaries 1 16 OD 250 µm ID Material nuts PEEK Polyether ether ketone Material fittings PEEK seals stainless steel rings StainlessSteelConnectionKit Dimensions...

Page 18: ...ool 2Alignmentnuts UserManual Please contact us if you have any questions or if you need help with your Fluidic Connect We wish you lots of succes with your microfluidic experiments Fluidic Connect 4515 User Manual V1 5 18 2009 Micronit Microfluidics www micronit com ...

Page 19: ...ith 10 years of experience in microfluidic chip manufacturing for science and industry Micronit is the perfect partner to outsource your microfluidic chip needs Make up to 4 different designs of desired channel layouts based on the standard 10 hole pattern Our design experts will transform them to CAD designs We will send the designs back to you for a final check and after your approval we will st...