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ALIBR
ATION
3 Select Height and check the central positioning of the needle by using the
buttons Move up and Move down. Select Position and adjust by using the
Move back and Move fwd buttons. Select Done after making necessary
adjustments.
4 Select Save to save the new configuration.
5.1.1.4 Calibration of the z-axis (step 3/5)
1 Select Calibrate to proceed with calibration.
Figure 5.12: Calibration 1 Step 3/5: check needle position above the chill rack.
2 If you haven’t done so yet, place a Chill 15 Rack onto the MACS MiniSampler.
Place three 15 mL conical centrifugation tubes in positions A1, B1 and C1.
Confirm be pressing Done. The needle arm will move to rack position A1.
3 Press Height and check the positioning of the uptake needle at the bottom
of the tube. To do so, press Down until the needle hits the bottom and the
Down button disappears. Go up again by one increment. The needle should be
centered.
4 If the needle tip is not located directly in the middle of the conical tube, press
Position. Use the needle navigation buttons (Move back, Move fwd) to correct
the needle position. Press Done to continue with calibration.
5 Press Save to store the new settings.
