Miltenyi Biotec autoMACS Pro, User Manual

Page 1: ...autoMACS Pro Separator User manual Version 5 Original instructions...

Page 2: ...GmbH in the preparation of this user manual However Miltenyi Biotec GmbH shall not be liable for any technical or editorial errors or omissions contained herein or for incidental or consequential dama...

Page 3: ...mbH Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 0 Fax 49 2204 85197 macs miltenyibiotec de www miltenyibiotec com Version 5 EN Original instructions autoMACS Pro Separ...

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Page 5: ...result in damage to the instrument Improper usage could also cause severe personal injury death unpredictable results instrument malfunction and premature wear of components shortening the lifetime of...

Page 6: ...or off 73 3 1 Switching on the instrument 73 3 1 1 When switching on the instrument for the first time 73 3 1 1 1 Installing the pump syringe 75 3 1 1 2 Installing autoMACS Columns 76 3 1 1 3 Set the...

Page 7: ...gn one labeling option to several samples 91 4 3 2 3 Apply an assigned labeling option to other samples 91 4 3 3 Assigning sample volumes 91 4 3 4 Assigning a cell separation program 92 4 3 5 Assignin...

Page 8: ...ograms 114 6 4 2 Maintenance programs 114 6 5 Preparation of single cell suspensions 115 6 6 Adjusting sample volumes 116 6 7 Selecting the appropriate tube rack 118 6 8 Monitoring the separation proc...

Page 9: ...of fuses 141 8 Quality Control 143 8 1 Cell recovery 143 8 2 Purity of isolated cell population 144 8 3 Viability of the cells 144 9 Troubleshooting 145 9 1 Issues with moving hardware 145 9 2 Hardwar...

Page 10: ...Technical data and specifications 155 10 1 Technical data and specifications 155 10 2 Compliance with FCC rules 157 10 3 EC declaration of conformity 158 11 Technical support 159 12 Limited warranty...

Page 11: ...er not specified by the manufacturer protection may be impaired At all times local working area safety instructions laboratory policies and standards regarding laboratory health and safety and prevent...

Page 12: ...rd and any actions that have to be taken Risk of electric shock Strong magnetic field Persons carrying pacemakers or electronic medical implants must maintain distance Risk of crushing and shearing of...

Page 13: ...tor Figure 1 Hazard sources and the position of the safety symbols on the front of the autoMACS Pro Separator Figure 2 Position of the safety symbols on the back of the autoMACS Pro Separator Risk of...

Page 14: ...tor is used if liquids are spilled into the instrument if an object has entered the instrument through the ventilation slots or if an object has been dropped into the instrument If flames or smoke app...

Page 15: ...devices that comply to UL 60950 are allowed to be connected to the RS232 connector labeled COM The connector labeled RS232 AUX is not in use In addition only original autoMACS Pro Separator equipment...

Page 16: ...powerful light emitting diodes LEDs for illuminating the reading area and the fluid bottles as well as the waste bottle Do not remove the bottle holders According to the international standard IEC 62...

Page 17: ...lug and do not use the instrument Failure of parts containing biohazardous material or liquids that have been in contact with such material could cause a hazard Columns tubes and any other consumables...

Page 18: ...ur end of life Miltenyi Biotec products in accordance with the applicable WEEE and hazardous waste disposal legislation which may differ by country or region Electrical and electronic equipment may co...

Page 19: ...isungen ist die Sicherheit nicht gew hrleistet rtliche Arbeitsschutzbestimmungen Laborrichtlinien Sicherheitsnormen und Un fall verh tungsvorschriften m ssen auf jeden Fall beachtet werden Wenden Sie...

Page 20: ...zu erfahren Gefahr eines Stromschlags Starkes Magnetfeld Personen mit Herzschrittmacher oder elektronischen medizinischen Implantaten m ssen Abstand halten Quetsch und Schergefahr Gefahr durch optisc...

Page 21: ...Gefahrensymbole des autoMACS Pro Separator Instruments Figure 3 Gefahrenquellen und Gefahrensymbole auf der Vorderseite des autoMACS Pro Separators Figure 4 Gefahrensymbole auf der R ckseite des autoM...

Page 22: ...rs Eine potentielle Gefahrenquelle liegt auch im Betrieb eines ge ffneten zu Boden gefallenen oder besch digten Ger tes Ebenfalls sollte unbedingt vermieden werden dass Fl ssigkeit in das Ger t gelang...

Page 23: ...tes befinden Sollte es erforderlich sein die Stromzufuhr zu unterbrechen ziehen Sie den Netzstecker An die mit COM beschriftete RS 232 Buchse d rfen nur Peripherieger te ang eschlossen werden die UL...

Page 24: ...kann Ihre Augen sch digen Das Ger t ist auch mit einem 2D Code Leser ausgestattet der einen sichtbaren Halbleiterlaser als Target Pointer Zeiger zur Einstellung der Leseposition verwen det sowie leis...

Page 25: ...vermeiden Sch tzen Sie auch Ihren Mund und Nase da Aerosole aus undichten Stellen des Systems austreten k nnten Mangelhafte oder unzureichende Schutzausr stung kann den Bediener des Instrumentes gef h...

Page 26: ...stellen dass das Ger t sich in vorschriftsm igem Zustand befindet Nutzen Sie nur von Miltenyi Biotec empfohlenes Zusatzger t und Upgrades zu Ihrem Ger t Fragen Sie Ihren rtlichen Miltenyi Biotec Vertr...

Page 27: ...L ndern eigene R cknahmeprogramme f r Altger te an und arbeitet weltweit mit lizensierten Partnern zusammen die an bestehende Recycling und Entsorgungssysteme angeschlossen sind Miltenyi Biotec erm g...

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Page 29: ...fabricante la seguridad se ver mermada En todo momento se debe observar la normativa nacional de seguridad en el tra bajo las normas del laboratorio y los est ndares de salud y seguridad en el la bora...

Page 30: ...s necesarias a realizar Riesgo de shock el ctrico Campo magn tico intenso Personas con marcapasos o implantes m dicos electr nicos deben mantenerse alejadas Riesgo de que se aplaste o corte alguna par...

Page 31: ...untos de peligro ylocalizaci n de s mbolos de seguridad que puede encontrar en la parte delantera del autoMACS Pro Separator Figure 6 Localizaci n de los s mbolos de seguridad en la parte transera del...

Page 32: ...impiar manualmente el separador autoMACS Pro Existe un riesgo potencial en caso de usar separador autoMACS Pro abierto que se haya ca do o que est averiado si se han derramado l quidos en el equipo si...

Page 33: ...situados tan cerca del operario como sea posible Si es necesario desconectar el suministro el ctrico desenchufe el cable de la toma de corriente Solamente los dispositivos perif ricos que cumplen con...

Page 34: ...star la posici n de lectura y diodos electroluminosos de gran alcance LED para iluminar el rea de la lectura las botellas de fluidos as como la botella de desechos Por favor no extraiga las cestas de...

Page 35: ...el sistema de fluidos todas las conexiones y tubos v lvulas columnas jeringas y agujas Si detectase alguna rotura o fuga sustituya todas las piezas da adas antes de encender el instrumento Si alguna...

Page 36: ...iene preguntas relativas a la descontaminaci n o el env o p ngase en contacto con el servicio de asistencia t cnica Informaci n sobre la Directiva de Residuos de Aparatos El ctricos y Electr nicos Was...

Page 37: ...AD EN IT FR ES DE Para m s informaci n o si desea eliminar su equipo de Miltenyi Biotec en el final de su vida til por favor p ngase en contacto con su representante local de Miltenyi Biotec o con el...

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Page 39: ...Les consignes de s curit concernant la zone de travail locale les bonnes pratiques de laboratoire ainsi que les directives relatives la sant la s curit et la pr vention des accidents en laboratoire do...

Page 40: ...choc lectrique Champ magn tique puissant Les personnes qui portent un stimulateur cardiaque ou un implant medical electronique doivent maintenir une distance de s curit Risque d crasement et de cisail...

Page 41: ...autoMACS Pro Figure 7 Les zones de risque et symboles de s curit sur la face avant du s parateur autoMACS Pro Figure 8 Les symboles de s curit sur la face arri re du s parateur autoMACS Pro Risque d c...

Page 42: ...nt rieur de l appareil D branchez le c ble lectrique avant de nettoyer manuellement le s parateur autoMACS Pro Un risque potentiel existe si le s parateur autoMACS Pro utilis est ouvert d form ou endo...

Page 43: ...de la valeur nominale du circuit de d rivation Assurez vous que l interrupteur principal et le connecteur du c ble lectrique soient facilement accessibles et plac s aussi pr s que possible de l op ra...

Page 44: ...rayonnement est invisible Ne pas regarder directement dans ce rayon au travers d instruments optiques p ex lentilles verres grossissants et microscopes Regarder dans l ouverture du VCSEL une distance...

Page 45: ...ns pour les substances utilis es Portez des gants de protection des v tements de protection et des lunettes de s curit afin d viter tout contact avec la peau et les yeux Prot ger aussi la bouche et le...

Page 46: ...iotec agr d effectuer tous les contr les de s curit requis par la proc dure de r paration afin de garantir que l appareil est parfaitement op rationnel Utilisez uniquement des options et extensions re...

Page 47: ...de nos produits des co organismes agr s travers le monde Miltenyi Biotec vous permet de recycler gratuitement vos quipements Miltenyi Biotec en fin de vie Les conditions et la disponibilit de cette of...

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Page 49: ...on forme alle istruzioni fornite dal fabbricante la sicurezza potrebbe risultare compromessa Vanno sempre osservate le istruzioni di sicurezza sul luogo di lavoro le procedure di laboratorio e le norm...

Page 50: ...zioni intraprendere Rischio di scosse elettriche Forte campo magnetico Portatori di pacemaker o dispositive medici elettronici devono mantenere una adeguata distanza di sicurezza Rischio di schiacciam...

Page 51: ...Figure 9 Punti critici e posizioni dei simboli di sicurezza sulla parte frontale del autoMACS Pro Separator Figure 10 Posizione dei simboli di sicurezza sulla parte posteriore del autoMACS Pro Separa...

Page 52: ...lire manualmente lo strumento autoMACS Pro Separator Esistono potenziali rischi in caso di utilizzo di uno strumento AutoMACS Pro Separator aperto caduto o danneggiato in caso di infiltrazione di liqu...

Page 53: ...e l 80 della potenza del circuito Accertarsi che l interruttore principale e l attacco del cavo dell alimentazione siano facilmente accessibili e posti quanto pi vicino possibile all operatore dello s...

Page 54: ...n osservare direttamente il laser con strumenti ottici p es lenti lenti di ingrandimento e microscopi Osservando la porta VSCL a una distanza inferiore a 100mm con uno strumento ottico si potrebbero d...

Page 55: ...ti e occhiali di protezione per prevenire il contatto con la pelle e gli occhi Proteggere sempre la bocca ed il naso dagli aereosol che possono provenire dal sistema L impiego di dispositivi di protez...

Page 56: ...rettamente Si usino solo opzioni ed aggiornamenti raccomandati da Miltenyi Biotec Si chiedano informazioni al rappresentante locale di Miltenyi Biotec in merito al servizio esteso di strumenti Milteny...

Page 57: ...E in tutto il mondo Miltenyi Biotec consente di riciclare le apparecchiature Miltenyi Biotec gratuitamente Le condizioni e la disponibilit di questa offerta variano da zona a zona a causa di differenz...

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Page 59: ...sequent cell analysis Labeling with MACS MicroBeads and fluorochrome conjugated antibodies can be performed simultaneously which further decreases handling times MACS MicroBeads are non toxic biodegra...

Page 60: ...the application The autoMACS Pro Separator features automated sample labeling autolabeling sample loading elution of the non labeled negative as well as the labeled positive cell fractions Up to six...

Page 61: ...ator Figure 1 2 Back view of the autoMACS Pro Separator Bottle Sensor 2D Code Reader plug External CAN Power socket Robotic needle arm Touchscreen Main switch Syringe pump 2D code reader Rack detector...

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Page 63: ...entative for assistance Do not place the autoMACS Pro Separator on an unstable table cart stand tripod or bracket As a consequence the instrument might fall down This may cause serious bodily harm and...

Page 64: ...per compartment autoMACS Pro Buffer Combination Separate box One year warranty Table 2 1 Components included in delivery 2 2 Materials required for operation For daily operation the following solution...

Page 65: ...compounds The MACS Bleach Solution is used in combination with the Safe program for disinfection of the fluidic system For special disinfection procedures contact Miltenyi Biotec Technical Support Pro...

Page 66: ...and 50 mL tubes 130 092 953 Chill 50 Racks 3 Chill Racks for 5 15 and 50 mL tubes 130 097 037 Chill 5 15 50 Set Set of three Chill Racks 130 097 038 MACS Reagent Rack 4 Accommodates 4 vials of MACS R...

Page 67: ...are required to lift the autoMACS Pro Separator The instrument must be gripped at the base of the orange bottle baskets located at both sides of the instrument The instrument is heavier at the front S...

Page 68: ...Stabilize the front of the instrument while lifting it Place the instrument onto a stable worktop surface and remove the plastic bag surrounding it Figure 2 4 Two people are required to lift the auto...

Page 69: ...e use e g MACS Bleach Solution 130 093 663 For proper disposal please follow your local regulations Operating the autoMACS Pro Separator requires Running Buffer Washing Solution and storage solution a...

Page 70: ...but do not remove the bottle closures from the bottles Do not disconnect the color coded tubing 3 Place a fresh bottle into its appropriate holder open it and fasten the bottle closure to the new bot...

Page 71: ...several different instruments e g the MACSQuant Instruments 1 Open the front door of the autoMACS Pro Separator 2 Tilt the MACS MiniSampler and slide the guiding into the receiving slot until a resis...

Page 72: ...t to the socket labeled External CAN at the rear panel of the instrument Fasten the screws of the connecter using the screwdriver included in the starter kit 7 Secure the MACS Reagent Rack 4 onto the...

Page 73: ...CS MiniSampler Keep the protective cover attached and closed during operation Note The protective cover is sensor controlled Running a separation is not possible if the protective cover is open Figure...

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Page 75: ...ec service provider or local power company Do not use extension cords or power strips Do not overload an electrical outlet The overall system load must not exceed 80 of the branch circuit rating Make...

Page 76: ...ee figure 4 3 Also depending on the menu and the instrument s status not all buttons are available at all times If a button is unavailable its backgroung color changes from white to grey After initial...

Page 77: ...he syringe at the dilutor valve by turning the top screw clockwise until a resistance is met see section 7 2 3 for a detailed description on how to clean and install the pump syringe Unfasten the top...

Page 78: ...column exchange from the Detail panel 5 Select Run to start the Col_ex program 6 Wait until the instrument prompts you to exchange the autoMACS Columns 7 Using both hands take the top and bottom of c...

Page 79: ...its slot and repeat steps 8 through 11 13 Ensure that the tubing is neither pinched nor obstructed 14 Press Done The program will then proceed to wash the columns with autoMACS Running Buffer Check t...

Page 80: ...ct the desired field and use the numeric keypad to modify the date and or time 4 Press Ok to return to the Option menu The procedure can be cancelled at anytime by pressing Cancel 3 1 1 4 Testing the...

Page 81: ...not carry enough buffer for at least one separation or if the waste bottle is full However the exact filling status cannot be determined Thus if more than one separation is to be performed check the...

Page 82: ...symbols for all fluid bottles are green except for the grey symbol for the storage solution bottle 5 Ensure that the symbol for the columns is green If the symbol is red exchange the columns The fill...

Page 83: ...tails 1 Press the shutdown button at the upper right hand corner of the screen see figure 3 9 2 Select Yes from the pop up dialog window 3 Upon completion of the Sleep program you will be prompted to...

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Page 85: ...he instrument s status not all buttons are available at all times If a button is unavailable its backgroung color changes from white to grey Menu Function Reagent To define the position of MACS Reagen...

Page 86: ...1 Color coded symbols indicate the status of the hardware components For further information on a particular component touch the symbol A pop up window will open and detail more information To close...

Page 87: ...minutes Figure 4 3 Sample status during separation 4 1 2 Fluid bottle status The status of fluid bottles 2 is indicated by color coded graphic symbols If the fluid bottles are full and the waste bott...

Page 88: ...tails on column exchange The level of the green fill on the column symbol indicates the remaining operation life of the autoMACS Columns Touch the column symbol to open a pop up window for further inf...

Page 89: ...er was detected 4 2 The Reagent menu The Reagent menu is used to assign reagent vials to one of the four reagent rack slots R1 R2 R3 and R4 to perform automated magnetic labeling Enter reagents manual...

Page 90: ...2D code reader Ensure that the 2D code is facing the blinking light The optimal reading distance is 0 5 2 5 cm from the code reader tilt the vial as depicted Figure 4 9 Scanning a reagent using the 2...

Page 91: ...lates 5 After all desired reagents are entered open the MicroBead reagent vials and place them on the MACS Reagent Rack 4 in the correct order 6 Go to the Separation menu to proceed with programming a...

Page 92: ...assigned positions delete an individual reagent as described above 2 Press Delete All 4 3 The Separation menu The Separation menu schematically represents a sample rack For each sample rack position i...

Page 93: ...menu 4 3 2 1 Assign a labeling option to a single sample 1 Highlight a sample using the sample rack template 2 Scroll through the Labeling submenu using the arrows 3 Select the desired Labeling reage...

Page 94: ...for a detailed explanation of the various cell separation strategies 1 Highlight one or more sample s using the sample rack template 2 Scroll through the Separation submenu using the arrows 3 Select a...

Page 95: ...cess was either aborted or some problem occurred See section 9 3 for details Figure 4 13 The Log list menu 1 Go to Log list and highlight a log from the log list 2 Press Details A detailed view of the...

Page 96: ...am is to be used with MACS Bleach Solution for cleaning and disinfection of the autoMACS Pro Separator Depending on the level of instrument use it is recommended to run the Safe program at least every...

Page 97: ...Performing a system check up The Check_up program allows the user to perform a system check up It is recommended to use the program if hardware errors occur Refer to section 9 1 for details O_bcr 2D...

Page 98: ...display the number of separations that have been performed on the autoMACS Pro Separator 1 Go to Option User settings Sepcount 2 Press Run A dialog box will report the number of separations 3 Press OK...

Page 99: ...iSampler or one of the needles was exchanged needle calibration should also be checked Calibr_2 automatically calibrates the liquid volume control This is crucial for correct measurement and processin...

Page 100: ...en prompted place the MACS Reagent Rack 4 containing four sample vials onto the MACS MiniSampler Confirm by pressing Done 4 A pop up window will appear Test position washing station Select Test positi...

Page 101: ...in the washing station arrow 5 When done with testing the current position press Done The next position NEG needle will be addressed automatically Figure 5 4 Test position NEG needle in the washing st...

Page 102: ...espectively Ensure that the uptake needle is positioned directly above the center of the tube Lower the needle all the way to the bottom of the tube to check that the needle is centered For the Reagen...

Page 103: ...5 calibration steps will begin namely the needle position in the washing station Figure 5 8 Calibration 1 Step 1 5 check needle position in the washing station 5 1 1 2 Calibrate the washing station Up...

Page 104: ...save the new configuration 5 1 1 3 Calibrate the washing station NEG needle step 2 5 1 Select Calibrate to proceed with calibration Figure 5 11 Calibration 1 Step 2 5 check NEG needle position in the...

Page 105: ...et place a Chill 15 Rack onto the MACS MiniSampler Place three 15 mL conical centrifugation tubes in positions A1 B1 and C1 Confirm be pressing Done The needle arm will move to rack position A1 3 Pres...

Page 106: ...se factory settings 4 Press Height and check the positioning of the uptake needle at the bottom of the tube To do so press Down until the needle hits the bottom and the Down button disappears Go up ag...

Page 107: ...r Press Done to confirm In the next steps you can test the calibration settings of the washing station the tube rack position A1 B1 and C1 the x position and vials 1 4 in the Reagent Rack To test a po...

Page 108: ...me 1 Go to Option User settings Calibr_2 Press Run to start the program 2 Press Calibrate The calibration is performed automatically Figure 5 15 Calibration 2 Step 1 1 3 Press Save to finish Calibr_2...

Page 109: ...can be performed manually or using the instrument s autolabeling feature The pre set separation programs of the autoMACS Pro Separator have been developed for optimal separation results independent fr...

Page 110: ...marker The primary antibody can either be unconjugated biotinylated or fluorochrome conjugated Second cells are magnetically labeled with MACS MicroBeads which either bind to the primary antibody or...

Page 111: ...in the autoMACS Column and will be eluted at the end of the separation process MACS Cell Isolation Kits for untouched isolation contain a cocktail of titrated antibodies and MACS MicroBeads for indire...

Page 112: ...d as the negative fraction into the negative fraction collection tube i e row B of the tube rack After automated retraction of the magnet the magnetically labeled cells are eluted as the positive frac...

Page 113: ...ial positive selection Special program for isolation of erythrocytes infected with Plasmodium falciparum 6 3 2 Depletion programs When running any depletion program the magnetically labeled non target...

Page 114: ...raction Volume of labeled fraction i e positive fraction Loading rate Possel 2 mL sample volume 2 mL 4 mL min Possels 2 mL sample volume 2 mL 1 mL min Posseld 2 mL sample volume 0 5 mL 4 mL min column...

Page 115: ...ve selection standard mode POSSELD2 Double positive selection POSSEL_S Positive selection sensitive mode POSSELD Double positive selection low elution volume POSSELDS Double positive selection sensiti...

Page 116: ...separations of rare cells e g stem cells the separation of cells from different species and is mandatory between whole blood separations Clean An optional very extensive rinsing program It uses stora...

Page 117: ...ning Buffer should be used during sample handling steps to minimize the risk of cell aggregation Resuspend cells carefully after centrifugation Large cell aggregates may interfere with the separation...

Page 118: ...han 1 10 cells do not scale down the volumes but use the same volumes as indicated In the table below the dilution volumes account for the first step of labeling For manual labeling please refer to th...

Page 119: ...5 10 Direct MicroBeads mouse Positive selection or depletion 1 10 cells per 90 L 180 L 2 10 5850 L 6 5 10 Whole Blood MicroBeads Whole blood or bone marrow 1 Original volume 1 mL 4 mL Cell Isolation K...

Page 120: ...or your convenience Row D can hold round bottom tubes for further analyses Select the appropriate tube rack according to table 6 4 It is recommended to cool down the tube rack for 3 4 hours in a refri...

Page 121: ...bar The status bar also displays the overall progress in minutes Color Program status Yellow Yet to be processed Purple Currently undergoing autolabeling Orange No autolabeling manual labeling White P...

Page 122: ...paration Red Error Check screen for error detection Purple Program Sleep is completed Switch off autoMACS Pro Separator Blinking Action required Check screen for required action Table 6 6 Fluid bottle...

Page 123: ...ash Now You now have the option to perform a quick rinse Qrinse or full rinse Rinse 7 Select Rinse and press Run to start the priming process The progress will be displayed at the bottom of the touchs...

Page 124: ...sk the user to check and confirm the filling status before proceeding to separation Press Continue to proceed 6 10 2 Cell separation after manual labeling 1 Go to Separation menu 2 Highlight the desir...

Page 125: ...reagent rack positions 2 Press Save Template 3 Allocate a name to the template 4 Press Ok 6 10 3 2 Saving a separation template 1 In the Separation menu configure the sample template 2 Press Save Tem...

Page 126: ...low definition of any programs beyond this position Alternatively press the shutdown button on the upper right hand corner of the screen and confirm Upon completion of the Sleep program the fluid bott...

Page 127: ...minated tissues and swabs according to their biological security level Three service contracts are available for the autoMACS Pro Separator Full Service Premium Full Service and Planned Maintenance Pl...

Page 128: ...ethanol isopropyl alcohol or MACS Bleach Solution followed by distilled or deionized water 3 Remove the needle guard that is attached underneath the outlet port holder Push up the ring holding the the...

Page 129: ...ew the top column connector while holding the column over the bottle to catch any fluids 9 Insert one end of a fresh column into the bottom column connector and gently screw in the column by turning i...

Page 130: ...hese are not the same as filters used for cell culture 7 2 3 Clean the pump syringe Depending on the level of usage the pump syringe must be cleaned every 1 3 months Appropriate maintenance and long t...

Page 131: ...user to switch the instrument off replace the syringe and tighten the plunger screw Press OK to confirm 6 Switch off the instrument using the main switch 7 Unscrew the syringe from the dilutor valve h...

Page 132: ...ak 11 Pull the plunger out of the syringe until it reaches its fitting in the plunger holder The plunger must have the same orientation as the fitting see figure 7 2 Tighten the plunger lock screw Fig...

Page 133: ...rocedure requires a column exchange 1 Switch off and unplug the instrument 2 Make sure that the needle arm is in the uppermost position 3 Swivel the front cover to the right side swivel the cover of t...

Page 134: ...iohazardous material they should be autoclaved after use Bottle closures can be autoclaved Neither autoclave the washing station nor wash it in a dishwasher In case of spillage it is recommended to us...

Page 135: ...tart the program Select valves that have to be exchanged Valves will automatically be turned to exchange position Note In case the valve exchange program Valve_ex cannot be performed e g valve does no...

Page 136: ...port locks or blind screws from the respective valve Figure 7 11 Unscrew the valve cover screw and detach tubing and valve port locks or blind screws 6 Loosen the two valve screws using the screwdrive...

Page 137: ...in the valve drive is positioned horizontally and in the lower half of the axis Figure 7 14 Left Valve plate driving section Right Rear view of the autoMACS Pro Separator valve 8 Make sure that the br...

Page 138: ...ct the tubing with the installed new valve and fasten tubing finger tight 13 Plug in and switch on the autoMACS Pro Separator 14 Perform a Rinse program and check the valves visually for leakage and a...

Page 139: ...of the coupling 6 Insert the new valve Contact Tech Support for assistance 7 When the dilutor valve is properly inserted mount the hexagonal socket screws 8 Connect the tubing according to the letter...

Page 140: ...tion Take care to clean spilled fluids with ethanol or disinfectant Figure 7 20 Dismantle the washing station Figure 7 21 Dismantle the washing station 11 Carefully remove the waste distributor from i...

Page 141: ...disinfectant Figure 7 23 The draining tube is attached 13 Install the waste distributor back to its former position and reinstall the washing station 14 Install the syringe pump 15 Switch on the autoM...

Page 142: ...ibutor 4 If necessary pull out the bottom cover as depicted in figure 7 9 5 Press clamps on both sides of the pump and pull out the pump head Figure 7 25 Detachment of the pump head 6 This will uncove...

Page 143: ...hand Make sure not to overtighten the screw 6 Plug in and switch on the instrument Run the program Rinse and check for leakage 7 Run the Calibr_2 program to calibrate the volume control of the instru...

Page 144: ...142 Figure 7 26 Removing the fuse holder 4 Push the fuse holder back into the housing and reconnect the main power cord...

Page 145: ...ugation steps OR to magnetic separation Also take counting statistics into consideration The standard deviation when counting cells is N N1 2 Therefore cell counting might be associated with large sta...

Page 146: ...scopy Trypan blue crosses the cell membrane of dead cells and stains the cells Live cells are not stained Propidium iodide 5 0 g mL is most often used for flow cytometry and fluorescence microscopy It...

Page 147: ...ardware If an error with moving hardware components occurs it is recommended to run the Check_up program The program automatically analyzes the functionality of moving hardware components A report is...

Page 148: ...4 If the problem persists contact Technical Support 9 2 2 Tubing leakage 1 Identify the location of the leaky tubing by running the Rinse program Press Wash Now from the lower menu bar in the Separati...

Page 149: ...ing operation the correct proceeding of a separation will be impaired Check all tubings that are connected to the fluid bottles Make sure that all tubings are fastened properly If a screw thread is da...

Page 150: ...ing off the instrument Decontaminate the instrument Refer to section 7 2 5 for details If the problem persists call Technical Support 9 2 8 MACS MiniSampler does not move properly Check whether the gu...

Page 151: ...Undo the tubing connector at the negative port and place into a 50 mL tube 4 Take out the uptake port needle from the needle holder and place it into a 50 mL tube 5 Undo the tubing connector of the wa...

Page 152: ...l number and details of the error message at hand i e error number module number file and error line If this is not possible please go to the instrument log list and view the log list details taking c...

Page 153: ...plunger could not be initialized Syringe not mounted correctly syringe damaged or diluter valve not positioned correctly Check if syringe is fastened correctly Exchange if broken or damaged If error i...

Page 154: ...er of the washing station is properly closed Remove any objects hindering the movement of the needle If needle hits rim of tube or top of rack calibrate needle arm using Calibr_1 program Press Lift fo...

Page 155: ...f column clogging blocked tubing set or any other cross section constriction during output of the negative fraction The negative fraction has not been eluted completely Negative cells are still remain...

Page 156: ...el Check for leakage at transition of needle to tubing and tubing to bubble sensor 7036 Plunger movement blocked because of column clogging blocked tubing set or any other cross section constriction d...

Page 157: ...specifications USA and Canada only UL listed and KAM cord minimum type SJ minimum 18 AWG 3 conductors Rated for a minimum temperature of 60 C Provided with grounding type NEMA 5 15P attachment plug r...

Page 158: ...n 3 TXD Pin 5 GND Pin 7 RTS Pin 8 CTS RS232 Interface labeled RS232 AUX Not in use Pins 1 4 6 7 8 9 NC Pin 2 RXD Pin 3 TXD Pin 5 GND RS232 Interface DC Output labeled RS232 BCR Pins 4 6 NC Pin 1 Input...

Page 159: ...uency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in...

Page 160: ...s Description Laboratory equipment Model autoMACSTM Pro Separator The Machinery complies with all essential requirements of the following directives 2014 30 EU Electromagnetic compatibility 2006 42 EC...

Page 161: ...the Technical Support team at Miltenyi Biotec headquarters Miltenyi Biotec GmbH Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 830 Fax 49 2204 8306 89 macstec miltenyibio...

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Page 163: ...ided by Miltenyi Biotec it does not cover Product issues not arising out of defects in material or workmanship including but not limited to Product issues resulting from failure to follow installation...

Page 164: ...iltenyi Biotec shall not be liable for any incidental or consequential damages for breach of any express or implied warranty or condition on this Product Some states or jurisdictions do not allow the...

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Page 166: ...Biotec Norden AB Scheelev gen 17 223 70 Lund Sweden macs miltenyibiotec se Customer service Sweden Phone 0200 111 800 Fax 046 280 72 99 Customer service Denmark Phone 80 20 30 10 Fax 46 46 280 72 99 C...