7
7 Maintenance
125
7.1
Daily maintenance
125
7.1.1
Instrument priming
125
7.1.1.1
Automatic instrument priming at startup
126
7.1.2
Clean uptake/outlet ports
126
7.2
Periodic maintenance
127
7.2.1
Column exchange
127
7.2.2
Exchange of hydrophobic air filters
128
7.2.3
Clean the pump syringe
128
7.2.4
Cleaning the washing station
131
7.2.5
Instrument disinfection
132
7.3
Corrective maintenance
133
7.3.1
Valve exchange
133
7.3.2
Dilutor valve exchange
136
7.3.3
Exchange of the peristaltic pump head
140
7.3.4
Exchange of tubing system
141
7.3.5
Exchange of fuses
141
8
Quality Control
143
8.1
Cell recovery
143
8.2
Purity of isolated cell population
144
8.3
Viability of the cells
144
9 Troubleshooting
145
9.1
Issues with moving hardware
145
9.2
Hardware issues not indicated by an error screen
146
9.2.1
Column leakage
146
9.2.2
Tubing leakage
146
9.2.3
Pump syringe leakage
147
9.2.4
Pump syringe is filled with air during operation
147
9.2.5
Washing station overflow
147
9.2.6
Outlet port is clogged
148
9.2.7
Contamination of tubing system
148
9.2.8
MACS MiniSampler does not move properly
148
9.2.9
Touchscreen remains dark
148
9.2.10
Disruption of power supply during cell separation
149
9.2.10.1 Rescue procedure A
149
9.2.10.2 Rescue procedure B
149
9.3
Hardware issues indicated by an error screen
150
