37091/04 – ch20 (Issued: 2020-06)
STEP
2: DEPLETION 3.1 – 3
STEP
2: DEPLETION 3.1
Sample parameter input
If the material check has been successful, the program continues
automatically with the query for di
ff
erent sample parameters
that are necessary to adjust the separation to each individual
sample and to provide the operator important information for
required bu
ff
er and bag volumes.
The window will display Screen 4-2.5 as shown.
1. Start by entering the WBC concentration of the sample. The
acceptable range is shown in the box on the bottom left
hand side. Press the “Undo” key to correct a wrong input.
Confirm that the value entered reflects the value of the
sample. Otherwise sample processing may not be optimal
(see “Important”).
To continue with the next input, press
ENT
2. Enter the percentage of labeled cells. Confirm that the
value entered reflects the value of the sample. Otherwise
sample processing may not be optimal (see “Important”).
To continue with the next input, press
ENT
3. Enter the final volume of the cell sample. Confirm that the
value entered reflects the value of the sample. Otherwise
sample processing may not be optimal (see “Important”).
To continue, press
ENT
Screen 4-2.5: Sample parameter input
Important
■
All sample parameters required refer
to the sample
post
cell labeling and
washing procedure.
■
The acceptable range shown in
the box on the bottom left hand
side always takes account of the
previous input. After each input the
software calculates and adjusts the
accepted range of the next query to
ensure the separation capacity of the
system will not be exceeded.
■
Specifying wrong parameters may
result in non-optimal sample pro-
cessing and may increase the risk of
lower depletion efficiency.
Entering
values for WBC concentration and/
or percentage of labeled cells which
are lower than the actual sample
values may result in lower depletion
efficiency due to the system being
overloaded. Entering a lower sample
volume may result in cell loss due to
overloading or even to incomplete
sample processing. If the specification
of any of the three parameters is too
high, processing time will increase
and the volume of the target cell
fraction will enlarge.
