37091/04 – ch20 (Issued: 2020-06)
STEP
3: CliniMACS® Tubing Set and CliniMACS Tubing Set LS – 9
STEP
3: CliniMACS® Tubing Set and CliniMACS Tubing Set LS
Attach CliniMACS® PBS/EDTA Buffer
The window will display Screen 4-3.9 as shown.
The prescribed bu
ff
er for CliniMACS® Plus Separations is
CliniMACS PBS/EDTA Bu
ff
er supplemented with HSA to a final
concentration of 0.5% (w/v).
1. Using aseptic techniques, remove the cap from the bu
ff
er
spike on the tubing set (see Figure 4-3.2) and connect it
to the bu
ff
er bag. Ensure that the septum is punctured,
allowing free flow of liquid. Gently squeeze the bag to
confirm that the spike has penetrated the bag.
If more than one liter bu
ff
er is necessary for a separation,
connect two bu
ff
er bags using a plasma transfer set.
2. Attach the bu
ff
er bag to the bu
ff
er bag hook on the bag
hanger (see Figure 4-3.8).
3. Adjust the height of the bu
ff
er bag hanger. Raise or lower
the bag hanger to accommodate the height to the size of
the bu
ff
er bag. Ensure that it is positioned high enough to
prevent severe bending of the tubing that could restrict
the flow, and that it is low enough to avoid the tubing or
connections being stretched (see Figure 4-3.8).
To proceed, press
ENT
Screen 4-3.9: Attach buffer bag
Note
The bag hangers are made for a
maximum load of 3 kg. Overloading the
bag hangers can cause damage to the
instrument.
Figure 4-3.8: Attach buffer bag
Buffer
bag hook
Bag hanger for
buffer bag
CliniMACS
PBS/EDTA Buffer
