37091/04 – ch20 (Issued: 2020-06)
STEP
3: CliniMACS® Depletion Tubing Set – 7
STEP
3: CliniMACS® Depletion Tubing Set
Attach CliniMACS® PBS/EDTA Buffer
The window will display Screen 4-3.7 as shown.
The prescribed bu
ff
er for CliniMACS® Plus Separations is
CliniMACS PBS/EDTA Bu
ff
er supplemented with HSA to a final
concentration of 0.5% (w/v).
1. Using aseptic techniques, remove the cap from the bu
ff
er
spike (see Figure 4-3.3) on the tubing set and connect it
to the bu
ff
er bag. Ensure that the septum is punctured,
allowing free flow of liquid. Gently squeeze the bag to
ascertain that the spike has penetrated the bag.
2. Attach the bu
ff
er bag to the bu
ff
er bag hook on the bag
hanger (see Figure 4-3.7).
3. Adjust the height of the bu
ff
er bag hanger. Raise or lower
the bag hanger to accommodate the height to the size of
the bu
ff
er bag. Ensure that it is positioned high enough to
prevent severe bending of the tubing that could restrict
the flow and that it is low enough to avoid the tubing or
connections being stretched (see Figure 4-3.7).
To proceed, press
ENT
Important
Due to the gravimetric rinsing steps, it is important that the
bu
ff
er bag is positioned higher than the Reapplication Bag and
the Non-Target Cell Bag (see Figure 4-3.8).
Screen 4-3.7: Attach buffer bag
Figure 4-3.7: Attach buffer bag
Buffer
bag hook
Bag hanger for
buffer bag
CliniMACS
PBS/EDTA Buffer
