37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
2 – STEP
4: CD34 SELECTION 1/2
Column wash I
The pre-column and separation column are washed extensively
to remove all unlabeled cells. Wash bu
ff
er is collected in the
Bu
ff
er Waste Bag. When ‘Column Wash I’ starts, the total
re maining time until the end of the separation procedure is
shown.
Release of cells I
The magnet is moved to the rear of the instrument (“OFF”
position). The retained cells are released at a high speed flow,
but the cells remain within an internal tubing cycle.
Reloading of cells I
The magnet is moved into the “ON” position again to magnetize
the separation column and the cells are reapplied on the
separation column.
Column wash II
Reloading of the cells is followed by a second washing step to
remove remaining unlabeled cells. Also all tubing are rinsed
several times.
Release of cells II, reloading of cells II, column wash III
The cells are released and reapplied on the separation column
for a second time in order to remove any unlabeled cells that
only stick to the column matrix. Afterwards the separation
column is washed again.
Release of cells III, reloading of cells III,
column wash IV
Additionally, the separation program CD34 SELECTION 2
includes a third release and reapplication step.
Final elution of the cells
The magnet is moved into the “OFF” position and the
magnetically labeled CD34 positive cells are released from the
separation column and collected in the attached Cell Collection
Bag.
