37091/04 – ch20 (Issued: 2020-06)
STEP 4: DEPLETION 3.1 – 3
STEP 4: DEPLETION 3.1
Disconnect bags and record process code
When the run has been completed, the window will display
Screen 4-4.3 as shown.
1. Record the process code.
2. Clamp or seal the tubing above the luer lock connecting
the Cell Collection Bag to the tubing set (see Figure 4-4.1).
Make three hermetic seals in the tubing directly below
valve no. 9. Carefully sever the middle seal to disconnect
the Cell Collection Bag from the tubing set.
3. Weigh the filled Cell Collection Bag. Record the weight.
Determine the weight of the target cell fraction by
subtracting the weight of the empty Cell Collection Bag
from the weight of the Cell Collection Bag containing the
target cells. Record the weight.
4. Mix the target cell suspension thoroughly by rotating the
bag. Take an aliquot of 0.5 mL and retain for analysis.
5. Using the heat sealer, seal o
ff
the tubing above the luer
lock of the Bu
ff
er Waste Bag (see Figure 4-4.1). Make three
hermetic seals in the tubing. Sever the center seal to
disconnect the Bu
ff
er Waste Bag.
6. Disconnect the Non-Target Cell Bag in the same way.
7.
Remove the Non-Target Cell Bag and the Bu
ff
er Waste
Bag. Keep all bags until final analysis of all cells has been
accomplished.
The target cells can now be processed in accordance with
clinical protocols.
To proceed, press
ENT
Screen 4-4.3: Disconnect bags and record
process code
Figure 4-4.1: Disconnect bags
Seal off Cell
Collection Bag
Seal off Buffer
Waste Bag
