Unexpected events
– DEPLETION 2.1
Target cells do not reach the Cell
Collection Bag.
Pump is unable to load the product from the Cell Preparation Bag
because the locking forceps next to the Cell Collection Bag is not open
during the cell loading sequence. Press ‘STOP’ and open locking forceps
next to Cell Collection Bag. Continue the depletion process by pressing
‘RUN’.
Allow the CliniMACS Plus Instrument to finish the separation program.
Do not abort current run, this may result in unnecessary cell loss. If a
part of the product remains in the Cell Preparation Bag after depletion
procedure has been finished, process the remaining cells with a new
tubing set, a new pre-system filter and su
ffi
cient new bu
ff
er.
Determine the total cell number, percentage of labeled cells, and sample
loading volume and enter actual sample parameters during the set-up
of the instrument (STEP 2) and start the new depletion.
Loading stopped before com-
plete sample has been loaded
onto the columns.
Air bubbles from the sample and/or pre-system filter activated the liquid
sensor before all of the sample had been loaded. Abort the current run
and check total cell number and depletion e
ffi
ciency of the cells in the
Cell Collection Bag. If necessary, process the remaining sample with a
new tubing set, a new pre-system filter and su
ffi
cient new bu
ff
er.
Determine the total cell number, percentage of labeled cells and sample
loading volume and enter actual sample parameters during the set-up
of the instrument (STEP 2) and start the new depletion.
Unexpected events
– DEPLETION 3.1
Loading stopped during
bulk
loading stage
before complete
sample has been loaded onto
the columns.
Pre-system filter inserted wrong way around. Therefore, the drip
chamber function is not available and air bubbles may pass the liquid
sensor directly causing the termination of the sample loading before
the complete sample has been applied. The instrument continues the
depletion process by directly beginning the next step. It is not possible
to restart the sample loading once it has stopped.
Abort the current run and pool the contents of the Reapplication Bag
and the Cell Preparation Bag. Process the remaining cells with a new
tubing set, a new pre-system filter and su
ffi
cient new bu
ff
er.
37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
5 – 8
