37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
2 – 8
rpm
Revolutions per minute
Sampling site coupler
Injection port, e.g., for removal of samples or addition of CliniMACS Reagents to
the Cell Preparation Bag
Selection bu
ff
er
See CliniMACS PBS/EDTA Bu
ff
er.
Selection column
See separation column.
Selection column holder
See separation column holder.
Separation column
Column in which magnetically labeled cells are separated when exposed to the
magnetic field
Separation column holder
Molded guides in the magnet housing that holds the separation column in place
Separation program
Software program designed for the enrichment or depletion of magnetically
labeled cell subsets from a mixed cell population. The operator can choose from a
menu of separation programs depending on the intended separation.
Separation reagent
Reagent for magnetic labeling of cells, e.g., CliniMACS CD34 Reagent
T-fitting
T-shaped fitting on a tubing set where three tubing meet
TCR
α
/
β
antigen
The TCR
α
/
β
is the T cell receptor heterodimer composed of two transmembrane
glycoprotein chains,
α
and
β
. Both chains are members of the lg superfamily and
consist of a constant and a polymorphic variable region. The variable region of
the TCR
α
/
β
is involved in recognition of antigenic peptides presented by the
MHC complex of antigen presenting cells. The TCR
α
/
β
antigen is expressed on the
majority of peripheral blood T cells.
Transfer bag
Bag with a tubing and a spike at the end
Wash Waste Bag
Collection bag in which the wash supernatant is collected by separation from the
sedimented cell suspension after centrifugation steps during sample preparation
WBC
White blood cells