37091/04 – ch20 (Issued: 2020-06)
Customer information
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3.8.1 Enrichment strategy
Enrichment of cells using the CliniMACS Tubing Set or CliniMACS Tubing Set LS
Figure 3.3: Strategy of enrichment programs using the CliniMACS Tubing Set or CliniMACS Tubing Set LS
S
N
Cell
Collection Bag
Negative
Fraction Bag
Magnet
Separation column
1. Separation
2. Elution
3. Enrichment strategy
Cell Preparation Bag
Cell
Preparation
Bag
Negative
Fraction Bag
Cell
Collection Bag
Separation
column
1. The magnet is in the “ON”-position.
Magnetically labeled cells are held in the
separation column, while other non-labeled
cells (non-target cells) flow through the
column and are collected in the Negative
Fraction Bag.
2. The magnet is in the “OFF”-position. The
magnetically labeled cells (target cells) are
released from the separation column and
collected in the Cell Collection Bag.
3. The
enrichment program
retains the magnetically labeled
cells in the separation column, the
non-labeled cells
(non-
target cells) flow through the column and are collected in
the
Negative Fraction Bag
. When the magnet is moved
into the “OFF”-position, the
magnetically labeled cells
(target cells) are released from the column and collected
in the
Cell Collection Bag
.
Note
The target cell fraction is always collected in the Cell Collection
Bag.
