37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
3 – 10
3.8.2 Depletion strategy
Depletion of cells using the CliniMACS Tubing Set LS – DEPLETION 2.1
Figure 3.4: Strategy of the depletion program DEPLETION 2.1 using the CliniMACS Tubing Set LS
S
N
1. Separation
2. Elution
3. Depletion strategy
Separation column
Cell
Preparation
Bag
Negative
Fraction Bag
Cell
Collection Bag
Separation
column
Cell Preparation Bag
Negative
Fraction Bag
Magnet
Cell
Collection Bag
1. The magnet is in the “ON”-position.
Magnetically labeled cells are held in
the separation column, while other non-
labeled cells (target cells) flow through
the column and are collected in the Cell
Collection Bag.
2. The magnet is in the “OFF”-position. The
magnetically labeled cells (non-target cells)
are released from the separation column
and collected in the Negative Fraction Bag.
3. The
depletion program
(DEPLETION 2.1) retains the
magnetically labeled cells in the separation column, the
non-labeled target cells
(target cells) flow through and
are collected in the
Cell Collection Bag
. When the magnet
is moved into the “OFF”-position, the
magnetically
labeled
cells
(non-target cells) are released from the
column and collected in the
Negative Fraction Bag
.
Note
The target cell fraction is always collected in the Cell Collection
Bag.
