II. Materials required
CliniMACS® Plus CD34 System components
-
CliniMACS® CD34 Reagent (REF 171-01)
Using the CliniMACS Plus CD34 System, one vial is required
for the normal scale application and two vials for the large
scale application.
-
CliniMACS Tubing Set (REF 161-01)
One CliniMACS Tubing Set must be used for the normal scale
application.
o r
CliniMACS Tubing Set LS (REF 162-01)
One CliniMACS Tubing Set LS must be used for the large
scale application.
-
CliniMACS PBS/EDTA Buffer (REF 700-25)
CliniMACS PBS/EDTA Buffer must be used for the cell pre-
paration and the CliniMACS Separation. For the preparation
procedure, two liters of buffer are required. For the
separation, one liter of buffer is required. Before use,
supplement the CliniMACS PBS/EDTA Buffer with HSA to a
final concentration of 0.5% (w/v).
Additional materials
-
Cell Preparation Bag
One 600 mL transfer bag, suitable for centrifugation, as well
as one sampling site coupler and two plasma transfer sets,
for use during the cell preparation procedure
-
Plasma Waste Bag and Wash Waste Bags
Three 600 mL transfer bags, suitable for centrifugation
-
Cell Collection Bag
One 150 mL transfer bag in combination with a Luer/Spike
Interconnector to connect the Cell Collection Bag to the
tubing set
- Pre-system filter
- Locking forceps
- Appropriate syringes (1 mL, 10 mL, 20 mL) and hypodermic
20 gauge needles
- Human serum albumin (HSA) to be added to the CliniMACS
PBS/EDTA Buffer to a final concentration of 0.5% (w/v)
- Sample tubes
Important
The application capacity for the
in
vitro
enrichment of CD34 positive cells
using the CliniMACS Plus CD34 System
amounts to 0.6
×
10
9
CD34 positive cells
out of a total cell number not exceeding
60
×
10
9
cells. For the
in vitro
enrichment
of up to 1.2
×
10
9
CD34 positive cells out
of a total cell number of 120
×
10
9
cells
(large scale application), two vials of the
CliniMACS CD34 Reagent are needed.
Note
Refer to chapter 3 for detailed information
regarding additional materials and
equip ment required.
37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
2 – STEP
1: CliniMACS® CD34 Reagent
