III. Preparative steps
Preparation of the CliniMACS® PBS/EDTA Buffer
Supplement three liters of CliniMACS® PBS/EDTA Bu
ff
er with
HSA to a final concentration of 0.5% (w/v), i.e., add 5 g HSA per
liter bu
ff
er.
Labeling and preparation of bags
- Label one 150 mL transfer bag as:
Cell Collection
. (This should
include patient identification, date and time of run, and
operator identification.) Insert a Luer/Spike Interconnector
into the port of the Cell Collection Bag. Place a locking
forceps on the tubing close to the bag and close the locking
forceps. Weigh the empty Cell Collection Bag with locking
forceps positioned close to the bag and the tubing lying on
the table next to the balance. Record the weight.
- Label one 600 mL transfer bag as:
Cell Preparation
. (This
should include patient identification, date and time of run,
and operator identification.) Insert a sampling site coupler
into the outside port of the Cell Preparation Bag. Place a
locking forceps on the tubing close to the bag and close the
locking forceps. Weigh the empty Cell Preparation Bag with
locking forceps positioned close to the bag and the tubing
hanging on the table next to the balance. Record the weight.
– Label one 600 mL transfer bag as:
Plasma Waste
. (This
should include patient identification, date, time of run, and
operator identification.)
- Label two 600 mL transfer bag as:
Wash Waste No. 1
and
Wash Waste No. 2
. (This should include patient identification,
date and time of run, and operator identification.)
Important
■
Note that
HSA is not a component
of the CliniMACS System
. Use only
pharmaceutical grade HSA approved
in your country. Carefully read the
package insert of the HSA used; in
particular the section regarding
hypersensitivity reactions and the
risk of infection that HSA as a blood-
derived product brings to all patients.
All risks arising from these materials
must be evaluated by the user.
■
Store the buffer for cell preparation
at +19 °C to +25 °C (+66 °F to +77 °F).
Lower or higher ambient temperature
will result in less purity and yield of
the target cells.
■
Since the length of the tubing can vary
during the preparation procedure, be
careful when determining the weight
of the Cell Preparation Bag. To acquire
an accurate reading confirm the
locking forceps are always positioned
close to the bag and are lying on the
balance and the rest of the tubing is
lying on the table next to the balance.
37091/04 – ch20 (Issued: 2020-06)
STEP
1: CliniMACS® CD34 Reagent – 3
STEP
1: CliniMACS® CD34 Reagent
